Measurement of the tensile strength of cell-biomaterial interface using the laser spallation technique.
Measurement of the tensile strength of cell-biomaterial interface using the laser spallation technique.
复制标题
使用激光散裂技术测量细胞-生物材料界面的拉伸强度。
DOI：
10.1016/j.actbio.2008.06.001
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发表时间：
2008
影响因子：
9.7
通讯作者：
V. Gupta
中科院分区：
文献类型：
--
作者：
Jaewoo Shim;E. Hagerman;Ben M. Wu;V. Gupta
先前开发的用于确定薄膜界面拉伸强度的激光散裂技术被成功地用于确定三种不同的活哺乳动物细胞（成骨细胞、软骨细胞和成纤维细胞）和聚苯乙烯（未经处理和纤维连接蛋白涂层）和钛表面之间界面的拉伸强度。在同一基质上的三种细胞类型之间，界面拉伸强度值没有明显差异，尽管在未经处理的聚苯乙烯表面上培养的成骨细胞的粘附强度略低。然而，对于不同表面处理的细胞，测量到显着差异。纤维连接蛋白的使用增加了所有细胞类型的界面拉伸强度，细胞与钛的结合比未处理的聚苯乙烯表面要好得多。有血清培养的细胞界面强度高于无血清培养。结果表明，激光散裂实验在确定局部界面微观结构和化学成分对细胞粘附的影响方面具有显著的灵敏度。
A previously developed laser spallation technique to determine the tensile strength of thin film interfaces was successfully adopted to determine the tensile strength of interfaces between three different live mammalian cells (osteoblast, chondrocyte and fibroblast) and polystyrene (untreated and fibronectin coated) and titanium surfaces. No noticeable differences in the interfacial tensile strength values were found across the three cell types on the same substrate although osteoblasts showed slightly lower adhesion strength when cultured on untreated polystyrene surfaces. Significant differences were, however, measured for cells treated on different surfaces. Use of fibronectin increased the interfacial tensile strength for all cell types, and cells bonded much better to titanium than to untreated polystyrene surfaces. Cell interfacial strength was higher when cultured with serum than in a serum-free environment. The results demonstrate the remarkable sensitivity of the laser spallation experiment in determining the effects of local interfacial microstructure and chemistry on cell adhesion.