Wolbachia infections are distributed throughout insect somatic and germ line tissues
Wolbachia infections are distributed throughout insect somatic and germ line tissues
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DOI：
10.1016/s0965-1748(98)00119-2
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发表时间：
1999-02-01
影响因子：
3.8
通讯作者：
O'Neill, SL
中科院分区：
文献类型：
--
作者：
Dobson, SL;Bourtzis, K;O'Neill, SL
沃尔巴克氏体是在许多节肢动物物种中形成母系遗传感染的细胞内微生物。这些细菌引起了广泛的关注，部分原因是它们在宿主中诱导的生殖改变，包括细胞质不相容性（CI），雌性化和孤雌生殖。虽然沃尔巴克氏体在昆虫生殖组织中的存在已经得到了很好的描述，但相对较少的研究已经检查了沃尔巴克氏体感染其他组织的程度。我们用Western印迹、斑点杂交和诊断PCR方法检测了Wolbachia在一些有代表性的昆虫宿主中的组织嗜性。这些研究的结果表明，沃尔巴克氏体在宿主组织中的分布比以前认识到的要广泛得多。此外，沃尔巴克氏体在体组织中的分布在不同的沃尔巴克氏体/宿主协会之间存在差异。一些协会显示，沃尔巴克氏体散布在大多数组织中，而另一些似乎更受限制，主要限于生殖组织。我们讨论了这些感染模式的相关性，沃尔巴克氏体/宿主共生体的演变和潜在的应用沃尔巴克氏体。(C)1999 Elsevier Science Ltd.保留所有权利。
Wolbachia are intracellular microorganisms that form maternally-inherited infections within numerous arthropod species. These bacteria have drawn much attention, due in part to the reproductive alterations that they induce in their hosts including cytoplasmic incompatibility (CI), feminization and parthenogenesis. Although Wolbachia's presence within insect reproductive tissues has been well described, relatively few studies have examined the extent to which Wolbachia infects other tissues. We have examined Wolbachia tissue tropism in a number of representative insect hosts by western blot, dot blot hybridization and diagnostic PCR. Results from these studies indicate that Wolbachia are much more widely distributed in host tissues than previously appreciated. Furthermore, the distribution of Wolbachia in somatic tissues varied between different Wolbachia/host associations. Some associations showed Wolbachia disseminated throughout most tissues while others appeared to be much more restricted, being predominantly limited to the reproductive tissues. We discuss the relevance of these infection patterns to the evolution of Wolbachia/host symbioses and to potential applied uses of Wolbachia. (C) 1999 Elsevier Science Ltd. All rights reserved.