Reconstruction of a bacterial isoprenoid biosynthetic pathway in Saccharomyces cerevisiae
Reconstruction of a bacterial isoprenoid biosynthetic pathway in Saccharomyces cerevisiae
复制标题
DOI：
10.1016/j.febslet.2008.10.045
复制
发表时间：
2008-12-10
期刊：
影响因子：
3.5
通讯作者：
Nielsen, Jens
中科院分区：
文献类型：
--
作者：
Maury, Jerome;Asadollahi, Mohammad A.;Nielsen, Jens
在大肠杆菌中转移和表达的真核甲羟戊酸途径，以及在酿酒酵母中重建的哺乳动物氢化可的松生物合成途径的例子表明，将代谢途径从一种生物体转移到另一种生物体可以对细胞特性产生强大的影响。在这项研究中，我们重建了酿酒酵母中大肠杆菌类异戊二烯的生物合成途径。编码该途径的七个酶步骤的基因被克隆并在酿酒酵母中表达。检测到来自七个基因的 mRNA，并且该途径被证明能够在其组成型类异戊二烯生物合成途径被抑制的条件下维持酵母的生长。 (C) 2008 年欧洲生化学会联合会。由 Elsevier B.V 出版。保留所有权利。
A eukaryotic mevalonate pathway transferred and expressed in Escherichia coli, and a mammalian hydrocortisone biosynthetic pathway rebuilt in Saccharomyces cerevisiae are examples showing that transferring metabolic pathways from one organism to another can have a powerful impact on cell properties. In this study, we reconstructed the E. coli isoprenoid biosynthetic pathway in S. cerevisiae. Genes encoding the seven enzymatic steps of the pathway were cloned and expressed in S. cerevisiae. mRNA from the seven genes was detected, and the pathway was shown able to sustain growth of yeast in conditions of inhibition of its constitutive isoprenoid biosynthetic pathway. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.