EPR detection of lipid-derived free radicals from PUFA, LDL, and cell oxidations
EPR detection of lipid-derived free radicals from PUFA, LDL, and cell oxidations
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DOI：
10.1016/s0891-5849(00)00407-x
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发表时间：
2000-09-15
影响因子：
7.4
通讯作者：
Buettner, GR
中科院分区：
文献类型：
--
作者：
Qian, SY;Wang, HP;Buettner, GR
我们用自旋陷阱5，5-二甲基吡咯烷-1-氧化物(DMPO)和顺磁共振(EPR)检测了脂类自由基(L-d(.)在多不饱和脂肪酸(PUFA)、低密度脂蛋白(LDL)和细胞(K-562和MCF-7)的过氧化过程中。从我们的可氧化靶中得到的所有DMPO氧中心自由基加合物的寿命都比未抽提时检测到的自旋加合物短(10小时)。在铁介导的DHA氧化中，我们观察到三个DMPO加合物在水相中，两个在有机相中。水相中含有DMPO/HO。A(N)近似于a(H)近似于14.8G)和两个碳中心自由基加合物(a(1)(N)近似于15.8G，a(1)(H)近似于22.6G；A(2)(N)近似于15.2G，a(2)(H)近似于18.9G)。有机相含有两个长链脂质自由基加合物(a(N)接近13.5G，a(H)接近10.2G；a(N)接近12.8G；a(H)接近6.85G，1.9G)。我们的结论是，提取显著延长了自旋加合物的寿命，允许用EPR检测各种脂类衍生的自由基。(C)2000年爱思唯尔科学公司。
We have used the spin trap 5,5-dimethyl-pyrroline-1-oxide (DMPO) and EPR to detect lipid-derived radicals (L-d(.)) during peroxidation of polyunsaturated fatty acids (PUFA), low-density lipoprotein (LDL) and cells (K-562 and MCF-7). All oxygen-centered radical adducts of DMPO from our oxidizable targets have short lifetimes (10 h) than the spin adducts detected without extraction. in iron-mediated DHA oxidation we observed three DMPO adducts in the aqueous phase and two in the organic phase. The aqueous phase contains DMPO/HO. a(N) approximate to a(H) approximate to 14.8 G) and two carbon-centered radical adducts (a(1)(N) approximate to 15.8 G, a(1)(H) approximate to 22.6 G; a(2)(N) approximate to 15.2 G, a(2)(H) approximate to 18.9 G). The organic phase contains two long-chain lipid radical adducts (a(N) approximate to 13.5 G, a(H) approximate to 10.2 G; and a(N) approximate to 12.8 G; a(H) approximate to 6.85 G, 1.9 G). We conclude that extraction significantly increases the lifetimes of the spin adducts, allowing detection of a variety of lipid-derived radicals by EPR. (C) 2000 Elsevier Science Inc.