Selective modulation of the functions of a conserved DNA motor by a histone fold complex.
Selective modulation of the functions of a conserved DNA motor by a histone fold complex.
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DOI：
10.1101/gad.259143.115
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发表时间：
2015-05-15
影响因子：
10.5
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Xue X;Choi K;Bonner JN;Szakal B;Chen YH;Papusha A;Saro D;Niu H;Ira G;Branzei D;Sung P;Zhao X
芽殖酵母Mph1解旋酶及其直系同源物驱动多个DNA交易。在这里，Xue等人表明，保守的组蛋白折叠MHF复合物促进Mph 1介导的受损复制叉的修复，但不影响DNA双链断裂修复的结果。芽殖酵母Mph1解旋酶及其直系同源物驱动多个DNA交易。阐明调节这些马达蛋白的机制对于理解基因组维持过程至关重要。在这里，我们表明，保守的组蛋白折叠MHF复合物促进Mph 1介导的修复受损的复制叉，但不影响DNA双链断裂修复的结果。从机制上讲，scMHF解除了染色体蛋白Smc5的结构维持对Mph1活动的抑制，这些活动与Mph1的复制相关修复有关，通过结合Mph1而不是DNA。因此，scMHF是Mph 1的功能特异性增强子，能够灵活响应不同的基因组修复情况。
Budding yeast Mph1 helicase and its orthologs drive multiple DNA transactions. Here, Xue et al. show that the conserved histone fold MHF complex promotes Mph1-mediated repair of damaged replication forks but does not influence the outcome of DNA double-strand break repair. Budding yeast Mph1 helicase and its orthologs drive multiple DNA transactions. Elucidating the mechanisms that regulate these motor proteins is central to understanding genome maintenance processes. Here, we show that the conserved histone fold MHF complex promotes Mph1-mediated repair of damaged replication forks but does not influence the outcome of DNA double-strand break repair. Mechanistically, scMHF relieves the inhibition imposed by the structural maintenance of chromosome protein Smc5 on Mph1 activities relevant to replication-associated repair through binding to Mph1 but not DNA. Thus, scMHF is a function-specific enhancer of Mph1 that enables flexible response to different genome repair situations.
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影响因子：
16
作者：
Wang, Yucai;Leung, Justin W.;Jiang, Yingjun;Lowery, Megan G.;Do, Huong;Vasquez, Karen M.;Chen, Junjie;Wang, Weidong;Li, Lei
通讯作者：
Li, Lei
影响因子：
3.8
作者：
Ede, Christopher;Rudolph, Christian J.;Kramer, Wilfried
通讯作者：
Kramer, Wilfried
影响因子：
2.6
作者：
Sugahara, R.;Mon, H.;Kusakabe, T.
通讯作者：
Kusakabe, T.
FANCM-MHF复合物的结构揭示了功能组装的物理特征
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
4.8
作者：
Chavez, Alejandro;Agrawal, Vishesh;Johnson, F. Brad
通讯作者：
Johnson, F. Brad