RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes
RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes
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DOI：
10.1074/jbc.m105303200
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发表时间：
2001-08-31
影响因子：
4.8
通讯作者：
Svejstrup, JQ
中科院分区：
文献类型：
--
作者：
Winkler, GS;Petrakis, TG;Svejstrup, JQ
拉长酶是一种与RNA聚合酶IL的拉长形式相关的组蛋白乙酰转移酶复合体。我们通过主要基于亲和层析的快速三步程序纯化了拉长酶。纯化的因子，holo-Elongator，是一个不稳定的六个亚基因子，由两个离散的亚复合物组成：一个由先前鉴定的Elp1， Elp2和Elp3蛋白组成，另一个由三个新的多肽组成，称为Elp4， Elp5和Elp6。编码新细长子蛋白的酵母菌基因的破坏与先前描述的其他help突变体的表型没有区别，并且在任何亚复合物中编码蛋白质的基因的破坏都不会产生新的表型。综上所述，我们的结果表明，holo-Elongator在体外和体内都是一个功能实体。Elp1和Elp3的后生动物同源物此前已有报道。我们克隆了酵母ELP4的人类同源基因，发现该基因在人体组织中普遍表达。
Elongator is a histone acetyltransferase complex that associates with the elongating form of RNA polymerase IL We purified Elongator to virtual homogeneity via a rapid three-step procedure based largely on affinity chromatography. The purified factor, holo-Elongator, is a labile six-subunit factor composed of two discrete subcomplexes: one comprised of the previously identified Elp1, Elp2, and Elp3 proteins and another comprised of three novel polypeptides, termed Elp4, Elp5, and Elp6. Disruption of the yeast genes encoding the new Elongator proteins confers phenotypes indistinguishable from those previously described for the other elp mutants, and concomitant disruption of genes encoding proteins in either subcomplex does not confer new phenotypes. Taken together, our results indicate that holo-Elongator is a functional entity in vitro as well as in vivo. Metazoan homologues of Elp1 and Elp3 have previously been reported. We cloned the human homologue of yeast ELP4 and show that this gene is ubiquitously expressed in human tissues.