Electrochemical detection of osmium tetroxide-labeled PCR-products by means of protective strands.
Electrochemical detection of osmium tetroxide-labeled PCR-products by means of protective strands.
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通过保护链电化学检测四氧化锇标记的 PCR 产物
DOI：
10.1016/j.talanta.2007.09.004
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发表时间：
2007
期刊：
影响因子：
6.1
通讯作者：
G.-U. Flechsig*
中科院分区：
文献类型：
--
作者：
T. Reske;M. Mix;H. Bahl;G.-U. Flechsig*
This communication reports about how single-stranded 136 base polymerase chain reaction (PCR) products labeled with electrochemically active osmium tetroxide bipyridine can be detected voltammetrically by hybridization with probe strands immobilized on gold electrodes. These electroactive ssDNA targets have been obtained by means of Lambda Exonuclease treatment of the double-stranded PCR products followed by hybridization of the remaining single strands with short protective strands and covalent labeling with osmium tetroxide bipyridine. Square-wave voltammetric signals of these osmium labels have been obtained only upon hybridization with the immobilized probe strands. An optimal 50°C hybridization temperature has been found with a saturation of the probe layer at 30min hybridization time and 7.5nmol/l target concentration. The blank capture probe layer alone did not yield any signal. Unprotected strands produced almost no interference. Such double-selective switch-on electrochemical hybridization assays hold great promise for the specific detection of PCR products.
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DOI：
10.1039/b106343j
发表时间：
2001-11
期刊：
The Analyst
影响因子：
--
作者：
J. Wang;A. Kawde;A. Erdem;M. Salazar
通讯作者：
J. Wang;A. Kawde;A. Erdem;M. Salazar
影响因子：
2.9
作者：
PALECEK, E;HUNG, MA
通讯作者：
HUNG, MA
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DOI：
--
发表时间：
2006
期刊：
影响因子：
--
作者：
B. Yosypchuk;M. Fojta;L. Havran;M. Heyrovský;E. Paleček
通讯作者：
E. Paleček
影响因子：
14.9
作者：
Kelley, SO;Boon, EM;Hill, MG
通讯作者：
Hill, MG
影响因子：
5
作者：
Pavel Kostečka;L. Havran;Hana Pivoňková;M. Fojta
通讯作者：
Pavel Kostečka;L. Havran;Hana Pivoňková;M. Fojta