LncRNA AGAP2-AS1 augments cell viability and mobility, and confers gemcitabine resistance by inhibiting miR-497 in colorectal cancer
LncRNA AGAP2-AS1 augments cell viability and mobility, and confers gemcitabine resistance by inhibiting miR-497 in colorectal cancer
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DOI：
10.18632/aging.102940
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发表时间：
2020-03-31
期刊：
影响因子：
5.2
通讯作者：
Li, Shiquan
中科院分区：
文献类型：
--
作者：
Hong, Sen;Yan, Zhenkun;Li, Shiquan
背景：最近，长非编码 RNA (lncRNA) 成为许多生物过程中的关键调节剂，例如胚胎发育、细胞生长和肿瘤发生。然而，lncRNA与结直肠癌（CRC）细胞增殖、转移和吉西他滨耐药之间的相关性尚不清楚。结果：AGAP2-AS1在CRC组织中过表达，与CRC患者的生存呈负相关。 AGAP2-AS1促进CRC细胞增殖并抑制细胞凋亡。此外，AGAP2-AS1增强了CRC细胞对吉西他滨的化疗耐药性。此外，AGAP2-AS1增强了CRC细胞的迁移和侵袭。机制研究表明，AGAP2-AS1 通过海绵 miR-497 在 CRC 进展中调节成纤维细胞生长因子受体 1 (FGFR1) 的表达。结论：我们确定了 AGAP2-AS1 在 CRC 发生和进展中的致癌作用。方法：采用 qRT-PCR 检测 AGAP2 反义 RNA 1 (AGAP2-AS1) 在 116 例 CRC 和 邻近的正常组织。荧光素酶报告基因测定用于检测 AGAP2-AS1 和 miR-497 之间的相互作用。采用异种移植肿瘤实验研究AGAP2-AS1的体内功能。
Background: Most recently, long non-coding RNAs (lncRNAs) emerge as crucial modulators in many biological processes, such as embryonic development, cell growth, and tumorigenesis. However, the correlations between lncRNAs and colorectal cancer (CRC) cell proliferation, metastasis, and gemcitabine resistance are not well understood.Results: The expression of AGAP2-AS1 was overexpressed in CRC tissues and negatively correlated with the survival of patients with CRC. AGAP2-AS1 promoted CRC cell proliferation and inhibited apoptosis. Moreover, AGAP2-AS1 enhanced the chemoresistance of CRC cells to gemcitabine. In addition, AGAP2-AS1 enhanced the migration and invasion of CRC cells. Mechanistic studies showed that AGAP2-AS1 regulated fibroblast growth factor receptor 1 (FGFR1) expression by sponging miR-497 in CRC progression.Conclusion: We identified an oncogenic role of AGAP2-AS1 in the development and progression of CRC.Methods: qRT-PCR was used to measure the expression of AGAP2 Antisense RNA 1 (AGAP2-AS1) in 116 cases of CRC and adjacent normal tissues. Luciferase reporter assays was used to detect the interaction between AGAP2-AS1 and miR-497. The xenograft tumor experiment was used to study the in vivo function of AGAP2-AS1.