Differential transport of cytosine-containing nucleosides by recombinant human concentrative nucleoside transporter protein hCNT1.
Differential transport of cytosine-containing nucleosides by recombinant human concentrative nucleoside transporter protein hCNT1.
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DOI：
10.1080/15257770008033018
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发表时间：
2000-01-01
影响因子：
1.3
通讯作者：
Cass, CE
中科院分区：
文献类型：
--
作者：
Graham, KA;Leithoff, J;Cass, CE
在转染的哺乳动物细胞中，研究了重组hCNT1对含胞嘧啶核苷的转运性。hCNT1介导的尿嘧啶、胞苷和脱氧胞苷转运的表观K-m值分别为59、140和150 μ m。500 μ m吉西他滨、阿糖胞苷和拉米夫定分别抑制尿嘧啶转运89%、32%和11%，这表明与吉西他滨（高亲和的hCNT1）不同，阿糖胞苷和拉米夫定是较差的hCNT1渗透剂。
The transportability of cytosine-containing nucleosides by recombinant hCNT1 was investigated in transfected mammalian cells. Apparent K-m values for hCNT1-mediated transport of uridine, cytidine and deoxycytidine were, respectively, 59, 140 and 150 mu M. Uridine transport was inhibited 89, 32 and 11%, respectively, by 500 CIM gemcitabine, cytarabine and lamivudine, demonstrating that, unlike gemcitabine (a high-affinity hCNT1 permeant), cytarabine and lamivudine are poor hCNT1 permeants.