Behavioral Deficits After Distal Focal Cerebral Ischemia in Mice: Usefulness of Adhesive Removal Test
Behavioral Deficits After Distal Focal Cerebral Ischemia in Mice: Usefulness of Adhesive Removal Test
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DOI：
10.1037/a0014157
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发表时间：
2009-02-01
影响因子：
1.9
通讯作者：
Boulouard, Michel
中科院分区：
文献类型：
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作者：
Freret, Thomas;Bouet, Valentine;Boulouard, Michel
大脑中动脉远端闭塞（dMCAo）是最常用的动物模型之一，它与人类中风非常相似。然而，尽管越来越多的人建议在临床前研究中评估组织学和功能结果，但后者往往被排除在外，因为很难评估，尤其是在小鼠中，行为障碍。我们的研究目的是深入筛选小鼠远端永久性MCAo的功能后果，以确定未来研究的相关行为。术后3周对两组小鼠进行感觉运动和认知测试。之后，通过组织染色或磁共振成像估计脑梗死。总的来说，虽然没有检测到长期的功能损伤，但粘合剂去除是唯一显示缺陷的测试。有趣的是，这种感觉运动损伤与手术后3周的皮质损伤相关。总之，尽管我们的大多数行为测试无法证明dmcao引起的缺陷，但作者表明，粘合剂去除测试是唯一一种足够敏感的测试，可以突出长期缺陷。因此，这一结果表明，如果使用相关的测试，该小鼠缺血模型可以通过组织学和行为学分析有效地评估治疗策略。
Distal occlusion of the middle cerebral artery (dMCAo), which closely mimics human stroke, is one of the most used animal models. However, although assessment of histological and functional outcome is increasingly recommended for preclinical studies, the latter is often excluded because of the high difficulties to estimate, especially in mice, behavioral impairments. The aim of our study was to deeply screen functional consequences of distal permanent MCAo in mice to target relevant behaviors for future studies. A set of sensorimotor and cognitive tests were performed during 3 weeks postsurgery in 2 groups of mice. Afterward, brain infarctions were estimated by histological staining or magnetic resonance imaging. Overall, while no long-term functional impairments could be detected, the adhesive removal was the only test showing a deficit. Interestingly, this sensorimotor impairment was correlated to cortical damage 3 weeks after surgery. In conclusion, despite the fact that dMCAo-induced deficits could not be evidenced by most of our behavioral tests, the authors showed that the adhesive removal test was the only one, sensitive enough, to highlight a long-term deficit. This result suggests therefore that this mouse model of ischemia is relevant to efficiently assess therapeutic strategies with histological but also behavioral analysis, provided that relevant tests are used.