Induction and origin of adventitious roots from chimeras of Brassica juncea and Brassica oleracea
Induction and origin of adventitious roots from chimeras of Brassica juncea and Brassica oleracea
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DOI：
10.1007/s11240-010-9686-8
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发表时间：
2010-02
期刊：
影响因子：
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通讯作者：
Xue-Yun Zhu;Shijie Chai;Li-ping Chen;Mingfang Zhang;Jingquan Yu
中科院分区：
文献类型：
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作者：
Xue-Yun Zhu;Shijie Chai;Li-ping Chen;Mingfang Zhang;Jingquan Yu
从嵌合体植物TCC（LI-LII-LIII = TCC; T =块茎芥菜，C =红甘蓝）的芽和叶诱导不定根，所述嵌合体植物TCC先前通过试管内嫁接块茎芥菜（芥菜）和红甘蓝（B. oleracea）。TCC茎段和叶片的不定根再生频率明显高于TTT和CCC。此外，培养基中α-萘乙酸的水平对TCC嵌合体生根效率的影响低于TTT和CCC。不定根数和鲜重分别为13.11根和0.274g，显著高于亲本。这表明，用不同的基因型替换组织发生LI层（最外面的顶端细胞层）可能会提高这些营养组织的不定根诱导能力，这是由于LI和其他两个组织发生层LII和LIII之间可能存在协同效应。经过聚合酶链反应分析和组织学研究，发现这些不定根起源于LIII组织发生层。
Adventitious roots were induced from shoots and leaves of the chimera plant TCC (LI-LII-LIII = TCC; T = Tuber mustard, C = Red Cabbage), previously developed by in vitro grafting of tuber mustard (Brassica juncea) and red cabbage (B. oleracea). The regeneration frequency of adventitious roots from TCC shoots and leaf sections was markedly higher than that obtained from the parents TTT (tuber mustard) and CCC (red cabbage). Moreover, levels of α-naphthaleneacetic acid in the culture medium had lower effects on rooting efficiency of TCC chimeras compared to those of TTT and CCC. The number and fresh weight of adventitious roots per TCC shoot, 13.11 roots and 0.274 g, respectively, were also significantly higher than those of the parents. This demonstrated that replacing the histogenic LI layer (the outermost apical cell layer) with a different genotype might improve adventitious root induction capability of these vegetative tissues due to likely synergistic effects between LI and the other two histogenic layers, LII and LIII. Following polymerase chain reaction analysis and histological investigation, it was found that these adventitious roots originated from the LIII histogenic layer.