Glucose tolerance and skeletal muscle gene expression in response to alternate day fasting
Glucose tolerance and skeletal muscle gene expression in response to alternate day fasting
复制标题
DOI：
10.1038/oby.2005.61
复制
发表时间：
2005-03-01
期刊：
影响因子：
--
通讯作者：
Ravussin, E
中科院分区：
文献类型：
--
作者：
Heilbronn, LK;Civitarese, AE;Ravussin, E
目的：隔日禁食可以延长啮齿动物的寿命，并且对于非肥胖人类来说短期内也是可行的。本研究的目的是检查 3 周隔日禁食对葡萄糖耐量和骨骼肌表达的影响，这些基因涉及脂肪酸转运/氧化、线粒体生物合成和应激反应。 研究方法和程序：在基线和 22 天隔日禁食（36 小时）后，测试非肥胖受试者（8 名男性和 8 名女性；BMI，20 至 30 kg/m(2)）对标准膳食的葡萄糖和胰岛素反应快）。在基线和第 21 天（禁食 12 小时）时从一组受试者 (n = 11) 中获取肌肉活检。结果：治疗 3 周后，女性对膳食的血糖反应略有受损 (p < 0.01)，但胰岛素反应没有变化。然而，男性的葡萄糖反应没有变化，胰岛素反应显着降低（p < 0.03）。尽管观察到 CPT1 表达增加的趋势，但参与线粒体生物合成或脂肪酸转运/氧化的基因表达没有显着变化 (p < 0.08)。隔日禁食后 SIRT1 mRNA 表达增加 (p = 0.01)。讨论：隔日禁食可能会对非肥胖女性的葡萄糖耐量产生不利影响，但不会对非肥胖男性产生不利影响。基因表达结果表明脂肪酸氧化和线粒体生物发生不受隔日禁食的影响。然而，SIRT1 表达的增加表明隔日禁食可以提高抗应激能力，这是热量限制啮齿动物的一个常见特征。
Objective: Alternate day fasting may extend lifespan in rodents and is feasible for short periods in nonobese humans. The aim of this study was to examine the effects of 3 weeks of alternate day fasting on glucose tolerance and skeletal muscle expression of genes involved in fatty acid transport/oxidation, mitochondrial biogenesis, and stress response.Research Methods and Procedures: Glucose and insulin responses to a standard meal were tested in nonobese subjects (eight men and eight women; BMI, 20 to 30 kg/m(2)) at baseline and after 22 days of alternate day fasting (36 hour fast). Muscle biopsies were obtained from a subset of subjects (n = 11) at baseline and on day 21 (12-hour fast).Results: Glucose response to a meal was slightly impaired in women after 3 weeks of treatment (p < 0.01), but insulin response was unchanged. However, men had no change in glucose response and a significant reduction in insulin response (p < 0.03). There were no significant changes in the expression of genes involved in mitochondrial biogenesis or fatty acid transport/oxidation, although a trend toward increased CPT1 expression was observed (p < 0.08). SIRT1 mRNA expression was increased after alternate day fasting (p = 0.01).Discussion: Alternate day fasting may adversely affect glucose tolerance in nonobese women but not in nonobese men. The gene expression results indicate that fatty acid oxidation and mitochondrial biogenesis are unaffected by alternate day fasting. However, the increased expression in SIRT1 suggests that alternate day fasting may improve stress resistance, a commonly observed feature of calorierestricted rodents.