Leucobacter musarum subsp. musarum sp. nov., subsp. nov., Leucobacter musarum subsp. japonicus subsp. nov., and Leucobacter celer subsp. astrifaciens subsp. nov., three nematopathogenic bacteria isolated from Caenorhabditis, with an emended description of Leucobacter celer.
Leucobacter musarum subsp. musarum sp. nov., subsp. nov., Leucobacter musarum subsp. japonicus subsp. nov., and Leucobacter celer subsp. astrifaciens subsp. nov., three nematopathogenic bacteria isolated from Caenorhabditis, with an emended description of Leucobacter celer.
复制标题
DOI：
10.1099/ijsem.0.000523
复制
发表时间：
2015-11
影响因子：
2.8
通讯作者：
Hodgkin J
中科院分区：
文献类型：
--
作者：
Clark LC;Hodgkin J
从佛得角Santa Antao （CBX151T, CBX152T）和日本Kakegawa （CBX130T）采集的线虫中分离到3株革兰氏染色阳性、不规则杆状、不运动、不产孢子的细菌。根据16S rRNA基因序列相似性，菌株CBX130T、CBX151T和CBX152T属于白杆菌属。这种关联得到了化学分类数据的支持（细胞壁中的2,4-二氨基丁酸，主要呼吸醌类MK-10和MK-11，主要极性脂质磷脂酰甘油和二磷脂酰甘油，主要脂肪酸anteiso-C15: 0， anteiso-C17: 0和iso-C16: 0）。菌株CBX130T和CBX152T具有显著的共同特征。基于形态、生理、化学分类和生化分析，菌株CBX152T代表了Leucobacter属的一个新种，建议将其命名为Leucobacter musarum sp. nov（型菌株CBX152T = DSM 27160T = CIP 110721T）。提出了两个亚种：十一月白杆菌十一月亚种；musarum无性系种群。11 .（类型菌株CBX152T = DSM 27160T = CIP 110721T）；多糖类化合物无性系种群。11 .（型应变CBX130T = DSM 27158T = CIP 110719T）。第三个新菌株CBX151T与celer Leucobacter NAL101T具有遗传相似性，表明这些菌株属于同一物种。基于形态、生理、化学分类和生化上的差异，我们提出将该物种划分为两个新的亚种：celucobacter celer subsp。速”无性系种群。11 .（型菌株NAL101T = KACC 14220T = JCM 16465T）；astrifaciens无性系种群。11 .（菌株类型CBX151T = DSM 27159T = CIP 110720T），并修订celeuobacter的描述。
Three Gram-stain-positive, irregular-rod-shaped, non-motile, non-spore-forming bacteria were isolated from nematodes collected from Santa Antao, Cabo Verde (CBX151T, CBX152T) and Kakegawa, Japan (CBX130T). Based on 16S rRNA gene sequence similarity, strains CBX130T, CBX151T and CBX152T were shown to belong to the genus Leucobacter. This affiliation was supported by chemotaxonomic data (2,4-diaminobutyric acid in the cell wall; major respiratory quinones MK-10 and MK-11; major polar lipids phosphatidylglycerol and diphosphatidylglycerol; major fatty acids anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0). Strains CBX130T and CBX152T were found to share salient characteristics. Based on morphological, physiological, chemotaxonomic and biochemical analysis, strain CBX152T represents a novel species of the genus Leucobacter, for which the name Leucobacter musarum sp. nov. (type strain CBX152T = DSM 27160T = CIP 110721T) is proposed. Two subspecies of Leucobacter musarum sp. nov. are proposed: Leucobacter musarum sp. nov. subsp. musarum subsp. nov. (type strain CBX152T = DSM 27160T = CIP 110721T) and Leucobacter musarum sp. nov. subsp. japonicus subsp. nov. (type strain CBX130T = DSM 27158T = CIP 110719T). The third novel strain, CBX151T, showed genetic similarities with Leucobacter celer NAL101T indicating that these strains belong to the same species. Based on morphological, physiological, chemotaxonomic and biochemical differences it is proposed to split the species Leucobacter celer into two novel subspecies, Leucobacter celer subsp. celer subsp. nov. (type strain NAL101T = KACC 14220T = JCM 16465T) and Leucobacter celer subsp. astrifaciens subsp. nov. (type strain CBX151T = DSM 27159T = CIP 110720T), and to emend the description of Leucobacter celer .