Polysialic acid in human milk - CD36 is a new member of mammalian polysialic acid-containing glycoprotein
Polysialic acid in human milk - CD36 is a new member of mammalian polysialic acid-containing glycoprotein
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DOI：
10.1074/jbc.m300458200
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发表时间：
2003-04-18
影响因子：
4.8
通讯作者：
Kitajima, K
中科院分区：
文献类型：
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作者：
Yabe, U;Sato, C;Kitajima, K
神经细胞粘附分子和电压敏感钠通道a亚基是已知的哺乳动物中仅有的两个被α -2,8-链聚唾液酸修饰的分子。我们在人乳中发现了一种新的含多聚西亚糖蛋白，并将其鉴定为CD36，属于清道夫受体超家族B类成员。含多sia的聚糖链通过碱性处理被去除，而n -肽聚糖酶F消化没有去除，这表明牛奶CD36在o -链聚糖链上含有多sia。CD36的多唾液化不仅发生在人乳中，也发生在小鼠乳中。然而，人类血小板中的CD36不会被多唾液化。PolySia CD36在任何哺乳期的乳汁中分泌，在分娩后1个月达到峰值。由此可见，乳汁中CD36的多聚对新生儿的发育具有重要的保护和营养作用。
The neural cell adhesion molecule and the voltage-sensitive sodium channel a-subunit are the only two molecules in mammals known to be modified by alpha-2,8-linked polysialic acid (polySia). We found a new polySia-containing glycoprotein in human milk and identified it as CD36, a member of the B class of the scavenger receptor superfamily. The polySia-containing glycan chain(s) were removed by alkaline treatment but not by peptide: N-glycanase F digestion, indicating that milk CD36 contained polySia on O-linked glycan chain(s). Polysialylation of CD36 occurs not only in human milk but also in mouse milk. However, CD36 in human platelets is not polysialylated. PolySia CD36 is secreted in milk at any lactation stage and reaches peak level at 1 month after parturition. Thus, it is suggested that polySia of milk CD36 is significant for neonatal development in terms of protection and nutrition.