Mapping of the gene encoding the regulatory subunit RII alpha of cAMP-dependent protein kinase (locus PRKAR2A) to human chromosome region 3p21.3-p21.2.
Mapping of the gene encoding the regulatory subunit RII alpha of cAMP-dependent protein kinase (locus PRKAR2A) to human chromosome region 3p21.3-p21.2.
复制标题
将编码 cAMP 依赖性蛋白激酶调节亚基 RII α（PRKAR2A 位点）的基因映射到人染色体区域 3p21.3-p21.2。
DOI：
10.1006/geno.1998.5326
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
Jahnsen,T
中科院分区：
文献类型：
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作者：
Tasken,K;Naylor,SL;Solberg,R;Jahnsen,T
我们利用聚合酶链式反应和两个不同的体细胞杂交定位板的Southern杂交分析，确定了cAMP依赖蛋白激酶调节亚基RIIα基因在人类3号染色体上的定位。此外，对3号染色体定位小组的聚合酶链式反应分析显示，只有在含有3p21.3-p21.2区域的细胞系中才存在人RIIα特异性扩增产物。以斯坦福G3辐射杂交板为模板，通过聚合酶链式反应证实了PRKAR2A的定位。分析结果表明，PRKAR2A与D3S3334(LOD评分12.5)连锁最近，D3S1322E和D3S1581次之。
We have determined the chromosomal localization of the gene for the regulatory subunit RIIα of cAMP-dependent protein kinase (locus PRKAR2A) to human chromosome 3 using polymerase chain reaction (PCR) and Southern blot analysis of two different somatic cell hybrid mapping panels. Furthermore, PCR analysis of a chromosome 3 mapping panel revealed the presence of a human RIIα-specific amplification product only in cell lines containing the region 3p21.3–p21.2. The localization of PRKAR2A was confirmed by PCR mapping using the Stanford G3 Radiation Hybrid Panel as template. The results from this analysis demonstrated that PRKAR2A is most closely linked to D3S3334 (lod score 12.5) and flanked by D3S1322E and D3S1581.