Stomatin, flotillin-1, and flotillin-2 are major integral proteins of erythrocyte lipid rafts
Stomatin, flotillin-1, and flotillin-2 are major integral proteins of erythrocyte lipid rafts
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DOI：
10.1182/blood.v97.4.1141
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发表时间：
2001-02-15
期刊：
影响因子：
20.3
通讯作者：
Prohaska, R
中科院分区：
文献类型：
--
作者：
Salzer, U;Prohaska, R
脂筏是富含鞘脂和胆固醇的膜微区，其不溶于非离子去污剂，具有低浮力密度，并且优先含有脂质修饰的蛋白质，如糖基磷脂酰肌醇（GP 1）锚定蛋白。从人红细胞中分离出脂筏，并鉴定了主要蛋白质组分。除了GPI锚定蛋白外，发现最丰富的整合蛋白是远亲膜蛋白stomatin（条带7.2b）、flotillin-1和flotillin-2。Flotillins，已经被描述为神经元中的脂筏组分和A498肾细胞中的小窝相关蛋白，尚未被识别为红细胞组分。此外，它表明，主要的细胞骨架蛋白，血影蛋白，肌动蛋白，带4.1，带4.2，部分与脂筏，气孔蛋白和flotillins是作为独立组织的高阶低聚物，这表明这些复合物作为单独的支架组件在红细胞脂筏的细胞质面。
Lipid rafts are sphingolipid- and cholesterol-rich membrane microdomains that are insoluble in nonionic detergents, have a low buoyant density, and preferentially contain lipid-modified proteins, like glycosyl phosphatidylinositol (GP1)-anchored proteins. The lipid rafts were isolated from human erythrocytes and major protein components were identified, Apart from the GPI-anchored proteins, the most abundant integral proteins were found to be the distantly related membrane proteins stomatin (band 7.2b), flotillin-1, and flotillin-2. Flotillins, already described as lipid raft components in neurons and caveolae-associated proteins in A498 kidney cells, have not been recognized as red cell components yet. In addition, it was shown that the major cytoskeletal proteins, spectrin, actin, band 4.1, and band 4.2, are partly associated with the lipid rafts, Stomatin and the flotillins are present as independently organized high-order oligomers, suggesting that these complexes act as separate scaffolding components at the cytoplasmic face of erythrocyte lipid rafts.