Development of Bifidobacterium lactis Bb 12 on β‐(2,6)‐Linked Fructan‐Containing Substrate
Development of Bifidobacterium lactis Bb 12 on β‐(2,6)‐Linked Fructan‐Containing Substrate
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DOI：
10.1002/elsc.200420043
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发表时间：
2004-10
影响因子：
2.7
通讯作者：
S. P. Semjonovs;M. Marauska;R. Linde;M. Grube;P. Zikmanis;M. Bekers
中科院分区：
文献类型：
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作者：
S. P. Semjonovs;M. Marauska;R. Linde;M. Grube;P. Zikmanis;M. Bekers
植物来源的β-（2，1）-连接果聚糖（菊粉）和相关低聚糖（FOS）作为不可消化的碳水化合物，即，强效益生元，可刺激多种益生乳酸菌的生长，包括多种双歧杆菌菌株。然而，相关的微生物来源的β-（2，6）-连接果聚糖（莱万和FOS）在这方面几乎没有研究。因此，各种果聚糖的生物合成特性，即，菊粉、莱万、低聚果糖（FOS）和果聚糖糖浆（FS）作为葡萄糖替代物在MRS培养基中进行了测试，并比较了它们对商业菌株乳双歧杆菌Bb 12的影响。虽然葡萄糖是生长和生物量形成的优选底物，但FS在48 h发酵期间表现出相当的细胞生长（分别为8.4 × 107计数/mL和1.0 × 107计数/mL）和酸化能力（分别为84 °T和74 °T），以及乳酸的增加和乙酸形成的减少。乳酸双歧杆菌Bb 12不利用菊粉作为唯一的碳源，这是从细胞计数减少60%和生长培养基的不显著（0.1 pH单位）酸化来判断的，而莱万在48小时发酵期间提供了细胞计数和酸化（0.4 pH单位）的显著增加。FOS制剂似乎是该菌株令人满意的碳源，但与含葡萄糖或FOS的培养基相比，观察到较低的酸化能力（56 °T）和细胞计数（分别为2.6%和22%）。在莱万的温和乳酸水解（37 °C，pH 3.3，24 h）的条件下获得的产物提高了乳酸双歧杆菌Bb 12的细胞计数（7- 10%）和酸化能力（2.7倍）。
β‐(2,1)‐linked fructan of plant origin (inulin) and the related oligosaccharides (FOS) as non‐digestible carbohydrates, i.e., potent prebiotics, can stimulate the growth of various probiotic lactic acid bacteria, including a number of bifidobacteria strains. The related β‐(2,6)‐linked fructans of microbial origin (levan and FOS), however, have scarcely been investigated in this respect. Therefore, the bifidogenic properties of various fructans, i.e., inulin, levan, fructooligosaccharides (FOS) and fructan syrup (FS), were tested as glucose substitutes in MRS media and were compared concerning their effect on the commercial strain Bifidobacterium lactis Bb 12. Although glucose was the preferred substrate for growth and biomass formation, FS exhibited a comparable cell growth (8.4 × 107 counts/mL and 1.0 × 107 counts/mL, respectively) and acidification power (84 °T and 74 °T, respectively) during 48 h of fermentation, as well as an increase in lactic acid and decrease in acetic acid formation. Bifidobacterium lactis Bb 12 did not utilize inulin as a sole carbon source as judged from the 60 % decrease in cell count and the insignificant (0.1 pH unit) acidification of the growth medium, whereas levan provided a noticeable increase in cell count and acidification (0.4 pH units) during 48 h of fermentation. FOS preparation appeared to be a satisfactory carbon source for this strain, but lower acidification power (56 °T) and cell counts were observed as compared to glucose‐ or FOS‐containing media (2.6 % and 22 %, respectively). The products obtained under conditions of mild lactic acid hydrolysis of levan (37 °C, pH 3.3, 24 h) enhanced the cell count (7–10 %) and acidification power (by a factor of 2.7) of Bifidobacterium lactis Bb 12.