Demecolcine-assisted enucleation for bovine cloning.
Demecolcine-assisted enucleation for bovine cloning.
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DOI：
10.1089/clo.2006.8.61
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发表时间：
2006-03
期刊：
影响因子：
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通讯作者：
T. Tani;H. Shimada;Y. Kato;Y. Tsunoda
中科院分区：
文献类型：
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作者：
T. Tani;H. Shimada;Y. Kato;Y. Tsunoda
本研究表明，在ii期中期牛卵母细胞中，deecolcine治疗至少30分钟可产生膜突出。母体染色体团块被浓缩在突出物中，这使得移除母体染色体进行核转移（NT）变得容易。成熟促进因子活性，而不是丝裂原激活的蛋白激酶活性，在去焦碱处理期间卵母细胞中增加了30%。用去焦碱治疗后产生的4个NT囊胚移植，获得一头正常的健康小牛。去美可林处理没有增加NT卵母细胞发育成囊胚的潜力。本研究表明，化学辅助染色体去除对牛的克隆是有效的。
The present study demonstrated that demecolcine treatment for at least 30 min produces a membrane protrusion in metaphase II-stage bovine oocytes. The maternal chromosome mass is condensed within the protrusion, which makes it easy to remove the maternal chromosomes for nuclear transfer (NT). Maturation promoting factor activity, but not mitogen-activated protein kinase activity, increased up to 30% in oocytes during demecolcine treatment. One normal healthy calf was obtained after transfer of four NT blastocysts produced following demecolcine treatment. Demecolcine treatment did not increase the potential of NT oocytes to develop into blastocysts. The present study demonstrated that chemically-assisted removal of chromosomes is effective for bovine cloning.