Measuring DNA Translocation Forces through MoS 2 -Nanopores with Optical Tweezers
Measuring DNA Translocation Forces through MoS 2 -Nanopores with Optical Tweezers
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DOI：
10.1016/j.matpr.2017.09.182
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Sebastian Knust;Dennis Kreft;Roland Hillmann;Andreas J Meyer;Martina Viefhues;P. Reimann;D. Anselmetti
中科院分区：
文献类型：
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作者：
Sebastian Knust;Dennis Kreft;Roland Hillmann;Andreas J Meyer;Martina Viefhues;P. Reimann;D. Anselmetti
测量作用在单个带电聚合物（如DNA）上的微小力，在通过纳米孔的移位过程中，为移位力学和动力学提供了有价值的见解。为了提高灵敏度，超薄纳米膜和小的纳米尺寸的纳米孔直径都是期望的。在本文中，我们展示了独立的单层二硫化钼纳米膜的制备剥离通过湿和干转移。我们用氦离子显微镜将直径约为40 nm的纳米孔研磨到这些纳米膜中。最后，我们用光镊测量了通过这种纳米孔的受控DNA移位，并在50 mV的跨膜电压下确定了7 pN的移位力。
Measuring the minute forces acting on a single charged polymer, like DNA, during the translocation through a nanopore offers valuable insights into translocation mechanics and dynamics. For increased sensitivity, both ultra-thin nanomembranes and small nanometer-sized nanopore diameters are desirable. In this paper, we show the preparation of free-standing monolayer molybdenum disulfide nanomembranes by exfoliation by both wet and dry transfer. We milled nanopores with approximately 40 nm diameter into those nanomembranes with a helium ion microscope. Finally, we measured the controlled DNA translocation through such a nanopore with optical tweezers and determined a translocation force of 7 pN at a transmembrane voltage of 50 mV.