Temporal Control of Retroviral Transgene Expression in Newborn Cells in the Adult Brain
Temporal Control of Retroviral Transgene Expression in Newborn Cells in the Adult Brain
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DOI：
10.1016/j.stemcr.2013.06.003
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发表时间：
2013-08-06
影响因子：
5.9
通讯作者：
Jessberger, Sebastian
中科院分区：
文献类型：
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作者：
Braun, Simon M. G.;Machado, Raquel A. C.;Jessberger, Sebastian
神经干/祖细胞（NSPCs）在成年哺乳动物大脑的不同区域产生新的神经元。除了经典的基于转基因的方法，逆转录病毒介导的遗传操作经常用于研究调节神经系统中神经发生的机制。在这里，我们显示了他莫昔芬可调节的雌激素受体（ERT 2）基序与转录因子（即，ASCL 1和NEUROD 1）能够在体外和体内暂时控制成年小鼠NSPCs中的转基因表达。因此，这里描述的方法代表了一种多功能的策略，用于调节基因表达，以研究分裂细胞及其后代中的基因功能。
Neural stem/progenitor cells (NSPCs) generate new neurons throughout life in distinct areas of the adult mammalian brain. Besides classical transgenesis-based approaches, retrovirus-mediated genetic manipulation is frequently used to study mechanisms that regulate neurogenesis in the nervous system. Here, we show that fusion of a tamoxifen-regulatable estrogen receptor (ERT2) motif to transcription factors (i.e., ASCL1 and NEUROD1) enables temporal control of transgene expression in adult mouse NSPCs in vitro and in vivo. Thus, the approach described here represents a versatile strategy for regulating gene expression to study gene function in dividing cells and their progeny.