Changes in epithelial cell turnover and extracellular matrix in human small intestine after TPN
Changes in epithelial cell turnover and extracellular matrix in human small intestine after TPN
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DOI：
10.1016/s0022-4804(02)00094-x
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发表时间：
2003-02-01
影响因子：
2.2
通讯作者：
Luciano, L
中科院分区：
文献类型：
--
作者：
Groos, S;Reale, E;Luciano, L
背景。接受全肠外营养（TPN）的成人中出现的空肠粘膜萎缩和结构重塑被认为源于组织稳态的紊乱。本研究旨在检查（1）肠内和肠外营养患者之间上皮细胞的增殖和凋亡是否存在差异，以及（2）已知影响隐窝-绒毛轴细胞周转的细胞外基质（ECM）蛋白的分布模式在TPN后是否发生改变。分别在环氧半薄切片和石蜡切片上测定有丝分裂频率和增殖指数[使用抗Ki-67抗原(MIB 1)的抗体]。应用形态学技术和TUNEL测定来检测细胞凋亡事件。在冷冻切片上进行 IV 型胶原、层粘连蛋白、纤连蛋白、生腱蛋白和 VI 型胶原的免疫定位。结果。 TPN 后，细胞更新显着增强，而上皮细胞死亡显着减少。 TPN 和 EN 患者的比较揭示了 ECM 蛋白层粘连蛋白、纤连蛋白和腱蛋白沿隐窝绒毛轴的分布模式的差异。此外，TPN 后观察到 IV 型和 VI 型胶原蛋白的表达增加。结论。成人中的 TPN 与上皮细胞更新的变化以及 ECM 蛋白表达和/或定位的变化有关。因此，患者营养供应的反向途径可能会改变环境组织条件，这可能会影响肠上皮细胞和细胞外基质之间的相互作用。 (C) 2003 年爱思唯尔科学（美国）。
Background. The atrophy and architectural remodeling of the jejunal mucosa arising in adults receiving total parenteral nutrition (TPN) has been suggested to originate from a disturbance in tissue homeostasis. The present study aims at examining (1) whether there are differences in proliferation and apoptosis of epithelial cells between enterally and parenterally nourished patients and (2) whether the distribution pattern of extracellular matrix (ECM) proteins known to influence cell turnover along the the crypt-villus axis is changed after TPN.Methods. The mitotic frequency and the proliferation index [using an antibody against Ki-67 antigen (MIB 1)] were determined on epoxy semithin and paraffin sections, respectively. Morphological techniques and the TUNEL assay were applied to detect apoptotic events. Immunolocalization of collagen IV, laminin, fibronectin, tenascin, and collagen VI was performed on cryosections.Results. After TPN the cell renewal was significantly enhanced, while epithelial cell death was drastically reduced. The comparison of TPN and EN patients revealed differences in the distribution patterns of the ECM proteins laminin, fibronectin, and tenascin along the crypt-villus axis. Moreover, after TPN an increased expression of collagen types IV and VI was observed.Conclusions. TPN in human adults is associated with alterations in epithelial cell turnover and changes in expression and/or localization of ECM proteins. Thus, the inverted route of nutrient supply in patients might modify environmental tissue conditions, which may influence the interactions between intestinal epithelial cells and the extracellular matrix. (C) 2003 Elsevier Science (USA).