Expression of proteins that inhibit calcium oxalate crystallization in vitro in the urine of normal and stone-forming individuals.
Expression of proteins that inhibit calcium oxalate crystallization in vitro in the urine of normal and stone-forming individuals.
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在正常和结石形成个体的尿液中抑制体外草酸钙结晶的蛋白质的表达。
DOI：
10.1053/ajkd.2001.20594
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Marengo,SR
中科院分区：
文献类型：
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作者：
Hedgepeth,RC;Yang,L;Resnick,MI;Marengo,SR
临床上活跃的草酸钙(CaOx)尿石症的诱因尚不清楚。本研究探讨了体外抑制CaOx结晶的尿蛋白与CaOx尿石症发病率之间的关系。第一种假设是尿钙氧合酶结晶抑制物水平在临床活动性结石形成患者(SFS)和正常人之间存在差异。第二个假设是，较低水平的尿CaOx结晶抑制物导致男性CaOx尿石症的发病率是女性的两到三倍。这些假说是根据先前对正常人和结石形成患者尿液中α-胰蛋白酶抑制物三聚体(IATI-Trimer)表达的观察得出的。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法对正常志愿者(男24例，女19例)和CaOx-SFS(男26例，女16例)的空泡尿蛋白质进行了分离。免疫印迹加增强化学发光法检测IATI-三聚体、骨桥蛋白和凝血酶原；然后测定条带的相对密度。除IATI-三聚体(P≤0.026，约两倍)外，正常人和CaOx结石形成者尿中CaOx结晶抑制物的相对密度无差异。因此，在正常和CaOx结石形成个体之间，CaOx结晶抑制蛋白的水平似乎没有普遍的增加或减少。女性IATI-三聚体的相对密度约为男性的3倍(P≤0.001)。其他CaOx结晶抑制剂的相对密度差异很小，生理意义值得怀疑。这些数据不支持这样一种假设，即由于尿CaOx结晶抑制蛋白水平的普遍下降，男性CaOx尿石症的发病率更高。
The factors precipitating clinically active calcium oxalate (CaOx) urolithiasis are not known. This study examined the relationships between urinary proteins that inhibit CaOx crystallization in vitro and the incidence of CaOx urolithiasis. The first hypothesis is that levels of urinary CaOx crystallization inhibitors differ between clinically active stone formers (SFs) and normal individuals. The second hypothesis is that lower levels of urinary CaOx crystallization inhibitors contribute to the two- to threefold greater incidence of CaOx urolithiasis in males compared with females. These hypotheses were derived from previous observations on the expression of urinary inter-a-trypsin inhibitor trimer (IaTI-trimer) in normal and stone-forming individuals. The proteins of void urine samples from normal volunteers (24 males, 19 females) and CaOx-SFs (26 males, 16 females) were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immunoreactive IaTI-trimer, osteopontin, and prothrombin were detected by immunoblot plus enhanced chemiluminescence; the relative densities of the bands were then determined. With the exception of IaTI-trimer (P ≤ 0.026, approximately twofold), there was no difference in the relative densities of CaOx crystallization inhibitors in the urine of normal and CaOx stone-forming individuals. Thus, there does not appear to be a generalized increase or decrease in levels of CaOx crystallization inhibitory proteins between normal and CaOx stone-forming individuals. The relative density of IaTI-trimer was approximately threefold greater in females than in males (P ≤ 0.001). Differences in the relative densities of the other CaOx crystallization inhibitors were small and of questionable physiological importance. These data do not support the hypothesis that males have a greater incidence of CaOx urolithiasis because of a generalized decrease in urinary CaOx crystallization inhibitory protein levels.