Amphiphilic Graphene Quantum Dots as Self-Targeted Fluorescence Probes for Cell Nucleus Imaging
Amphiphilic Graphene Quantum Dots as Self-Targeted Fluorescence Probes for Cell Nucleus Imaging
复制标题
两亲性石墨烯量子点作为细胞核成像的自靶向荧光探针
DOI：
10.1002/adbi.201700191
复制
发表时间：
2018-08-01
影响因子：
4.1
通讯作者：
Wu, Minghong
中科院分区：
文献类型：
--
作者：
Wang, Liang;Wu, Bin;Wu, Minghong
本文报道了一步溶剂热法合成两亲性高荧光石墨烯量子点作为核靶向荧光探针。GQD与N和Cl配体的边缘官能化使它们具有两亲性和带正电荷，以促进细胞核中的膜穿过和组蛋白结合。Cl掺杂可以显著增强GQD的荧光性能，因此在优化的Cl掺杂下，其荧光量子产率提高到30%。自靶向CQD表现出用于可视化细胞核的精细结构（诸如核被膜和核仁）的细胞核成像能力，这可以与它们独特的表面特征（如两亲性和高正电荷）相关联。这些发现为癌症诊断和治疗提供了一种简单的核靶向策略。
Herein, a one-step solvothermal strategy to synthesize amphiphilic, highly fluorescent graphene quantum dots (GQDs) as nucleus-targeted fluorescence probes is reported. The edge-functionalization of GQDs with N and CI ligands renders them amphiphilic and positive charged for boosting membrane crossing and histone binding in the nuclei. The fluorescent properties of the GQDs can be markedly enhanced by CI doping, and thus their fluorescence quantum yield is raised to 30% under optimized CI doping. The self-targeted CQDs exhibit multicolor cell imaging capability for visualization of fine architectures of the nucleus such as the nuclear envelope and nucleoli, which can be associated with their distinct surface features like amphiphilicity and high positive charge. These findings serve as a simple nuclear targeting strategy for cancer diagnosis and therapy.