Improved protocols for the illumina genome analyzer sequencing system.
Improved protocols for the illumina genome analyzer sequencing system.
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DOI：
10.1002/0471142905.hg1802s62
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发表时间：
2009-07
影响因子：
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通讯作者：
Turner, Daniel J
中科院分区：
文献类型：
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作者：
Quail, Michael A;Swerdlow, Harold;Turner, Daniel J
在本单元中，我们描述了我们对标准Illumina协议所做的一系列改进，以使测序过程在高通量环境中更加可靠，减少扩增偏倚，缩小插入片段大小的分布，并可靠地获得高产量的数据。
In this unit, we describe a set of improvements we have made to the standard Illumina protocols to make the sequencing process more reliable in a high-throughput environment, reduce amplification bias, narrow the distribution of insert sizes, and reliably obtain high yields of data.