OsGA2ox5, a gibberellin metabolism enzyme, is involved in plant growth, the root gravity response and salt stress.
OsGA2ox5, a gibberellin metabolism enzyme, is involved in plant growth, the root gravity response and salt stress.
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OsGA2ox5 是一种赤霉素代谢酶，参与植物生长、根部重力响应和盐胁迫
DOI：
10.1371/journal.pone.0087110
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Cai W
中科院分区：
文献类型：
--
作者：
Shan C;Mei Z;Duan J;Chen H;Feng H;Cai W
赤霉素（GA）2-氧化酶通过2β-羟基化在GA分解代谢途径中发挥重要作用。有两类GA 2 ox，即，较大类别的C19-GA 2 ox和较小类别的C20-GA 2 ox。本研究克隆了水稻GA 2氧化酶基因OsGA 2 ox 5，并对其进行了功能鉴定。BLASTP分析表明，OsGA 2 ox 5属于C20-GA 2 oxs亚家族，即作用于C20-GAs（GA 12，GA 53）的GA 2 oxs亚家族。OsGA 2 ox 5-YFP在瞬时转化洋葱表皮细胞中的亚细胞定位揭示了该蛋白在细胞核和细胞质中的存在。实时荧光定量PCR分析沿着GUS染色结果表明，OsGA 2 ox 5基因在水稻的根、茎、叶、鞘和穗中均有表达。过量表达OsGA 2 ox 5的水稻植株表现出显性的矮秆和GA缺乏表型，茎短，生殖器官发育晚于野生型。通过施加浓度为10 µM的外源GA 3，部分挽救了侏儒症表型。OsGA 2 ox 5 cDNA在拟南芥中的异位表达导致了类似的表型。实时荧光定量PCR检测结果表明，GA合成相关基因和GA信号传导基因在转基因水稻中的表达量均高于野生型水稻;编码GA合成途径最后一步关键酶的OsGA 3 ox 1在转基因水稻中的表达量也高于野生型水稻。OsGA 2 ox 5-ox植株的根系表现出增加的淀粉粒积累和重力反应，揭示了GA在根系淀粉粒发育和重力反应中的作用。此外，过表达OsGA 2 ox 5的水稻和拟南芥植株比野生型植株更能抵抗高盐胁迫。这些结果表明，OsGA 2 ox 5在水稻的GA稳态、发育、重力反应和胁迫耐受性中起重要作用。
Gibberellin (GA) 2-oxidases play an important role in the GA catabolic pathway through 2β-hydroxylation. There are two classes of GA2oxs, i.e., a larger class of C19-GA2oxs and a smaller class of C20-GA2oxs. In this study, the gene encoding a GA 2-oxidase of rice, Oryza sativa GA 2-oxidase 5 (OsGA2ox5), was cloned and characterized. BLASTP analysis showed that OsGA2ox5 belongs to the C20-GA2oxs subfamily, a subfamily of GA2oxs acting on C20-GAs (GA12, GA53). Subcellular localization of OsGA2ox5-YFP in transiently transformed onion epidermal cells revealed the presence of this protein in both of the nucleus and cytoplasm. Real-time PCR analysis, along with GUS staining, revealed that OsGA2ox5 is expressed in the roots, culms, leaves, sheaths and panicles of rice. Rice plants overexpressing OsGA2ox5 exhibited dominant dwarf and GA-deficient phenotypes, with shorter stems and later development of reproductive organs than the wild type. The dwarfism phenotype was partially rescued by the application of exogenous GA3 at a concentration of 10 µM. Ectopic expression of OsGA2ox5 cDNA in Arabidopsis resulted in a similar phenotype. Real-time PCR assays revealed that both GA synthesis-related genes and GA signaling genes were expressed at higher levels in transgenic rice plants than in wild-type rice; OsGA3ox1, which encodes a key enzyme in the last step of the bioactive GAs synthesis pathway, was highly expressed in transgenic rice. The roots of OsGA2ox5-ox plants exhibited increased starch granule accumulation and gravity responses, revealing a role for GA in root starch granule development and gravity responses. Furthermore, rice and Arabidopsis plants overexpressing OsGA2ox5 were more resistant to high-salinity stress than wild-type plants. These results suggest that OsGA2ox5 plays important roles in GAs homeostasis, development, gravity responses and stress tolerance in rice.
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