Intervertebral disc regeneration after implantation of a cell-free bioresorbable implant in a rabbit disc degeneration model
Intervertebral disc regeneration after implantation of a cell-free bioresorbable implant in a rabbit disc degeneration model
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DOI：
10.1016/j.biomaterials.2010.03.078
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发表时间：
2010-08-01
期刊：
影响因子：
14
通讯作者：
Woiciechowsky, Christian
中科院分区：
文献类型：
--
作者：
Endres, Michaela;Abbushi, Alexander;Woiciechowsky, Christian
腰椎间盘退变是引起下腰痛的最常见原因。有趣的是，所有可用的治疗方法仅限于治疗症状，而不是椎间盘潜在的生物变化。将冷冻干燥的可吸收非织造聚乙醇酸(PGA)-透明质酸(HA)植入物用于新西兰白兔退行性腰椎间盘病(DDD)模型。将构建物浸泡在同种异体血清中，植入椎间盘缺损处。摘除腰椎间盘的动物仅作为对照。术后一周，两组手术椎间盘的T2加权/脂肪抑制序列信号强度均低于健康椎间盘。12个月后，植入组的信号强度比1周的结果增加了51%，而假手术组的信号强度从1周到12个月保持在相同的水平。12个月后的组织学和免疫组织化学定量检查显示细胞向缺损区迁移，并形成了椎间盘修复组织。对照组中，含有II型胶原的修复组织不明显。综上所述，椎间盘摘除后植入基于聚合物的支架可诱导组织再生，从而改善椎间盘的水分含量。(C)2010爱思唯尔有限公司。保留所有权利。
Degeneration of the intervertebral disc is the most common cause of lower back pain. Interestingly, all available treatments are limited to treat the symptoms and not the underlying biologic alterations of the disc. Freeze-dried resorbable non-woven polyglycolic acid (PGA) - hyaluronan implants were used in a degenerated disc disease (DDD) model in New Zealand white rabbits. The constructs were immersed in allogenic serum and implanted into the disc defect. Animals with discectomy only served as controls. The T2-weighted/fat suppression sequence signal intensity of the operated discs as assessed by magnet resonance imaging decreased in both groups one week after the operation compared to a healthy disc. After 12 months the implanted group showed an increase of 51% in the signal intensity compared to the 1-week results whereas the signal intensity in the sham group remained on the same level from one week to 12 months. Histological and quantitative immunohistochemical examination after 12 months indicated cell migration into the defect and showed formation of disc repair tissue. In controls, repair tissue containing type II collagen was not evident. In conclusion, the implantation of polymer-based constructs after discectomy induces tissue regeneration resulting in improvement of the disc water content. (C) 2010 Elsevier Ltd. All rights reserved.