Corneal wound healing in an osteopontin-deficient mouse
Corneal wound healing in an osteopontin-deficient mouse
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DOI：
10.1167/iovs.07-1007
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发表时间：
2008-04-01
影响因子：
4.4
通讯作者：
Saika, Shizuya
中科院分区：
文献类型：
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作者：
Miyazaki, Ken-ichi;Okada, Yuka;Saika, Shizuya
目的。目的：探讨骨桥蛋白(OPN)缺失在小鼠角膜损伤修复中的作用。方法：以野生型(WT)和OPN缺失(KO)小鼠眼部成纤维细胞为材料，研究OPN对细胞行为的影响。结果：在细胞培养中，OPN参与了细胞黏附和眼成纤维细胞的迁移。外源性OPN对角膜上皮细胞的黏附无影响。OPN在正在愈合的受损小鼠角膜中表达上调。单纯上皮清创术后，OPN的丢失不影响上皮愈合。与WT小鼠相比，KO小鼠角膜切口处损伤的愈合延迟，肌成纤维细胞的出现和转化生长因子β1的表达减少。缺乏OPN促进了碱烧伤后的组织破坏，导致KO小鼠的角膜穿孔发生率高于WT小鼠。结论OPN调节伤口愈合相关的成纤维细胞行为，并需要在伤口愈合后恢复角膜的生理结构。
PURPOSE. To investigate the effects of loss of osteopontin (OPN) in the healing of the injured cornea in mice. Cell culture study was also conducted to clarify the effects of OPN in fibroblast behaviors.METHODS. Ocular fibroblasts from wild-type (WT) and OPN-null (KO) mice were used to study the role of OPN on cell behavior. The effect of the lack of OPN on corneal wound healing was evaluated in mice.RESULTS. In cell culture, OPN is involved in cell adhesion and in the migration of ocular fibroblasts. Adhesion of the corneal epithelial cell line was not affected by exogenous OPN. OPN was upregulated in a healing, injured mouse cornea. Loss of OPN did not affect epithelial healing after simple epithelial debridement. Healing of an incision injury in cornea was delayed, with less appearance of myofibroblasts and transforming growth factor beta 1 expression in a KO mouse than in a WT mouse. The absence of OPN promoted tissue destruction after an alkali burn, resulting in a higher incidence of corneal perforation in KO mice than in WT mice.CONCLUSIONS. OPN modulates wound healing-related fibroblast behavior and is required to restore the physiological structure of the cornea after wound healing.