Characterization of the radish mitochondrialnad3/rps12 locus: analysis of recombination repeats and RNA editing
Characterization of the radish mitochondrialnad3/rps12 locus: analysis of recombination repeats and RNA editing
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DOI：
10.1007/bf02426961
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发表时间：
1996-05
期刊：
影响因子：
2.5
通讯作者：
C. Rankin;Marc Cutright;C. Makaroff
中科院分区：
文献类型：
--
作者：
C. Rankin;Marc Cutright;C. Makaroff
为了进一步研究植物线粒体DNA中低频重组和萝卜线粒体RNA编辑的相关序列，从萝卜的一个正常栽培品种和雄性不育Ogura细胞质中分离并鉴定了thenad 3/rps 12基因座。在这两个位点之间的断点处发现了一个与其他萝卜线粒体DNA重排有关的重复序列，表明它也参与了thenad 3/rps 12重排。与其他一些萝卜线粒体基因类似，nad 3/rps 12基因组序列已经包含了几个，但不是全部，通常在植物nad 3和rps 12基因中编辑的碱基。对nad 3/rps 12 cDNA的分析表明，mRNA没有被编辑。一个部分编辑的成绩单被确定出的22个检查。这一发现，沿着nad 3/rps 12 RNA以非常低的水平存在的观察，提出了萝卜线粒体可能不编码这些基因的功能拷贝的可能性。与此假设相一致，DNA印迹分析在细胞核中检测到snad 3/rps 12序列。
In order to further investigate sequences that are responsible for low-frequency recombination in plant mitochondrial DNAs and RNA editing in radish mitochondria, thenad3/rps12locus has been isolated and characterized from a normal cultivar of radish and the male-sterile Ogura cytoplasm. A repeated sequence that has been implicated in other radish mitochondrial DNA rearrangements was identified at the breakpoint between the two loci indicating that it was also involved in thenad3/rps12rearrangement. Similar to some other radish mitochondrial genes,nad3/rps12genomic sequences already contain several, but not all, of the bases that are typically edited in plant mitochondrialnad3andrps12genes. Analysis ofnad3/rps12cDNAs indicated that the mRNAs are not edited. One partially edited transcript was identified out of the twenty two that were examined. This finding, along with the observation thatnad3/rps12RNAs are present at very low levels, raises the possibility that radish mitochondria may not encode functional copies of these genes. Consistent with this hypothesis, DNA-blot analysis detectsnad3/rps12sequences in the nucleus.