DNA VACCINES - PROTECTIVE IMMUNIZATIONS BY PARENTERAL, MUCOSAL, AND GENE-GUN INOCULATIONS
DNA VACCINES - PROTECTIVE IMMUNIZATIONS BY PARENTERAL, MUCOSAL, AND GENE-GUN INOCULATIONS
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DOI：
10.1073/pnas.90.24.11478
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发表时间：
1993-12-15
影响因子：
11.1
通讯作者：
ROBINSON, HL
中科院分区：
文献类型：
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作者：
FYNAN, EF;WEBSTER, RG;ROBINSON, HL
表达流感病毒血凝素糖蛋白的质粒DNA已针对其引发针对相同亚型致死性流感攻击的保护性免疫的能力进行了测试。在使用在盐水中的50至300微克纯化DNA进行两次接种的试验中，67 - 95%的试验小鼠和25 - 63%的试验鸡已受到保护免受致死性流感攻击。实现良好保护的肠胃外接种途径包括肌肉内和静脉内注射。成功的黏膜接种途径包括向鼻孔或气管施用DNA滴液。到目前为止，最有效的DNA免疫是通过使用基因枪将涂有DNA的金珠递送至表皮来实现的。在小鼠中，通过用载有少至0.4微克DNA的金珠进行两次免疫，实现了95%的保护。支持成功DNA免疫的途径的广泛性，以及基因枪免疫所需的极少量DNA，凸显了这种极其简单的技术在亚单位疫苗开发方面的潜力。
Plasmid DNAs expressing influenza virus hemagglutinin glycoproteins have been tested for their ability to raise protective immunity against lethal influenza challenges of the same subtype. In trials using two inoculations of from 50 to 300 mug of purified DNA in saline, 67-95% of test mice and 25-63% of test chickens have been protected against a lethal influenza challenge. Parenteral routes of inoculation that achieved good protection included intramuscular and intravenous injections. Successful mucosal routes of vaccination included DNA drops administered to the nares or trachea. By far the most efficient DNA immunizations were achieved by using a gene gun to deliver DNA-coated gold beads to the epidermis. In mice, 95% protection was achieved by two immunizations with beads loaded with as little as 0.4 mug of DNA. The breadth of routes supporting successful DNA immunizations, coupled with the very small amounts of DNA required for gene-gun immunizations, highlight the potential of this remarkably simple technique for the development of subunit vaccines.