Pulling G-quadruplex Out of Dilemma for Better Colorimetric Performance
Pulling G-quadruplex Out of Dilemma for Better Colorimetric Performance
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DOI：
10.1016/j.snb.2021.129830
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Duan Yixiang
中科院分区：
文献类型：
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作者：
Huang Zhijun;Li Yinjun;Yao Naizhi;Duan Yixiang
The main cause was indentified for the poor colorimetric performance of PCR generated G-quadruplex. In order to realize simple and sensitive quantitative analysis, some improved colorimetric strategies are proposed, which can achieve significant colorimetric enhancement by promoting the unwinding of double-stranded products and enabling the correct folding of G-quadruplex. Subsequently, the simple strategy of adding DNA denaturant during colorimetric analysis was further combined with colony PCR to realize sensitive and specific detection of food-borne pathogen E.coli O157:H7. Because the visualized analysis of nucleic acids can be realized by just making minor adjustment to general PCR primers, the simple improved colorimetric strategy is expected to play an important role in nucleic acid related detection and diagnosis.
