Jolly SAD
Jolly SAD
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DOI：
10.1107/s090744490200118x
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发表时间：
2002-03-01
期刊：
影响因子：
--
通讯作者：
Dodson, E
中科院分区：
文献类型：
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作者：
Dauter, Z;Dauter, M;Dodson, E
基于单波长反常色散(SAD)的大分子晶体结构定相的例子表明，这种方法比预期的更强大，在结构生物学中可能有更广泛的应用。更好的数据收集设施和低温技术，再加上强大的数据处理、相位调整、密度修改和自动建模程序，意味着SAD方法可能会因为其简单性、对波长的要求不那么严格以及比多波长(MAD)方法更快的数据收集和相位而受到广泛欢迎。它可以在任何可以观察到异常散射的波长下进行，在许多情况下使用实验室X射线源。
Examples of phasing macromolecular crystal structures based on single-wavelength anomalous dispersion (SAD) show that this approach is more powerful and may have more general application in structural biology than was anticipated. Better data-collection facilities and cryogenic techniques, coupled with powerful programs for data processing, phasing, density modification and automatic model building, means that the SAD approach may gain wide popularity owing to its simplicity, less stringent wavelength requirements and faster data collection and phasing than the multi-wavelength (MAD) approach. It can be performed at any wavelength where anomalous scattering can be observed, in many cases using laboratory X-ray sources.