INVITRO INDUCTION OF RESISTANCE IN COAGULASE-NEGATIVE STAPHYLOCOCCI TO VANCOMYCIN AND TEICOPLANIN
INVITRO INDUCTION OF RESISTANCE IN COAGULASE-NEGATIVE STAPHYLOCOCCI TO VANCOMYCIN AND TEICOPLANIN
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DOI：
10.1093/jac/22.3.321
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发表时间：
1988-09-01
影响因子：
5.2
通讯作者：
WATANAKUNAKORN, C
中科院分区：
文献类型：
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作者：
WATANAKUNAKORN, C
将20株凝固酶阴性葡萄球菌（18株溶血葡萄球菌和2株表皮葡萄球菌）在含有万古霉素或替考拉宁亚抑菌浓度（最低抑菌浓度的一半）的肉汤培养基中连续传代培养。在含抗生素的肉汤中传代5次后，再次测定抗生素的最低抑菌浓度。然后将这些菌在含有新的最低抑菌浓度值一半的抗生素浓度的肉汤中再次传代培养。该实验在传代25次后终止。只有1株菌对万古霉素的最低抑菌浓度升高了4倍。另一方面，有16株菌对替考拉宁的最低抑菌浓度升高了4倍。
Twenty strains of coagulase-negative staphylococci (18 strains of Staphylococcus haemolyticus and two of Staphylococcus epidermidis) were serially subcultured in broth media containing subinhibitory concentrations (half the MIC) of either vancomycin or teicoplanin. The MIC of the antibiotic was again measured after five passages in antibiotic-containing broth. The organisms were then subcultured in broth containing antibiotic concentrations half of the new MIC value. The experiment was terminated after 25 passages. Only one strain developed a four-fold MIC increase in vancomycin MIC. On the other hand 16 strains developed four fold MIC increases to teicoplanin.