Overexpression of CRK13, an Arabidopsis cysteine-rich receptor-like kinase, results in enhanced resistance to Pseudomonas syringae
Overexpression of CRK13, an Arabidopsis cysteine-rich receptor-like kinase, results in enhanced resistance to Pseudomonas syringae
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DOI：
10.1111/j.1365-313x.2007.03064.x
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发表时间：
2007-05-01
期刊：
影响因子：
7.2
通讯作者：
Raina, Ramesh
中科院分区：
文献类型：
--
作者：
Acharya, Biswa R.;Raina, Surabhi;Raina, Ramesh
在所有生物体中，蛋白激酶在将信息从感知信号传递给效应基因方面发挥着重要作用。富含半胱氨酸的受体样激酶（CRKs）是植物受体样激酶（RLKs）的一个亚家族，有40多个成员在细胞外结构域含有新的C-X8-C-X2-C基序列（DUF26）。在这里，我们报告了该基因家族的一个成员，CRK13的分子特征。与强毒菌株相比，该基因对无毒菌株的表达诱导更快、更强，并在病原菌感染后4 h内达到表达高峰。在地塞米松（DEX）处理下，通过DEX诱导启动子表达CRK13基因的植物表现出病原体防御激活的所有测试特征，包括快速组织塌陷，高水平积累几种防御相关基因转录物，包括PR1、PR5和ICS1，以及水杨酸（SA）的积累。此外，与野生型col0相比，这些植物对毒性病原体的抑制作用约为20倍。crk13赋予的病原体抗性依赖于水杨酸。基因表达分析显示，过表达CRK13的植株与经Pst DC3000 （avrRpm1）处理的植株表达谱存在显著的重叠。我们的研究表明，上调CRK13可导致超敏反应相关的细胞死亡，并通过增加水杨酸的积累诱导对病原体的防御。
Protein kinases play important roles in relaying information from perception of a signal to the effector genes in all organisms. Cysteine-rich receptor-like kinases (CRKs) constitute a sub-family of plant receptor-like kinases (RLKs) with more than 40 members that contain the novel C-X8-C-X2-C motif (DUF26) in the extracellular domains. Here we report molecular characterization of one member of this gene family, CRK13. Expression of this gene is induced more quickly and strongly in response to the avirulent compared with the virulent strains of Pseudomonas syringae, and peaks within 4 h after pathogen infection. In response to dexamethasone (DEX) treatment, plants expressing the CRK13 gene from a DEX-inducible promoter exhibited all tested features of pathogen defense activation, including rapid tissue collapse, accumulation of high levels of several defense-related gene transcripts including PR1, PR5 and ICS1, and accumulation of salicylic acid (SA). In addition, these plants suppressed growth of virulent pathogens by about 20-fold compared with the wild-type Col-0. CRK13-conferred pathogen resistance is salicylic acid-dependent. Gene expression analysis using custom cDNA microarrays revealed a remarkable overlap between the expression profiles of the plants overexpressing CRK13 and the plants treated with Pst DC3000 (avrRpm1). Our studies suggest that upregulation of CRK13 leads to hypersensitive response-associated cell death, and induces defense against pathogens by causing increased accumulation of salicylic acid.