Development of a method of nasal secretions sampling for local nasal inflammation studies
Development of a method of nasal secretions sampling for local nasal inflammation studies
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DOI：
10.1080/1744666x.2023.2228493
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发表时间：
2023-06
影响因子：
4.4
通讯作者：
Xu-xiang Xu-Xu-xiang-Xu-90058220;Xu Zhang;Dongsen Liu;Kunpeng Wang;Yang Wang;Chengshuo Wang;Yuan Zhang;Jingyun Li;Lu Zhang
中科院分区：
文献类型：
--
作者：
Xu-xiang Xu-Xu-xiang-Xu-90058220;Xu Zhang;Dongsen Liu;Kunpeng Wang;Yang Wang;Chengshuo Wang;Yuan Zhang;Jingyun Li;Lu Zhang
摘要背景鼻腔分泌物中免疫标志物的分析在鼻部疾病的研究中具有重要意义。我们提出了一种改进的鼻分泌物采集和处理方法--棉片法。方法分别用传统海绵法和棉片法采集31例健康对照和32例鼻部疾病患者的鼻分泌物。检测与鼻部疾病相关的14种细胞因子和趋化因子的浓度。结果棉片法鼻腔分泌物的性质较海绵法均匀。疾病组棉片法采集的IL-6浓度显著高于对照组(P=0.002)，棉片法可区分对照组与疾病组的IL-1β阳性检出率(P=0.031)和肿瘤坏死因子-α阳性检出率(P=0.001)。鼻腔分泌物中炎症介质水平可初步区分不同的鼻部疾病。结论棉片法是一种无创、可靠的鼻腔分泌物采集方法，有利于检测鼻黏膜局部炎症反应和免疫反应。
ABSTRACT Background Analysis of immune markers in nasal secretions has become crucial in the study of nasal diseases. We proposed the cotton piece method, a modified method, for the collection and processing of nasal secretions. Methods The nasal secretions of 31 healthy control participants and 32 patients with nasal diseases were collected by the traditional sponge method and the cotton piece method, respectively. The concentrations of 14 cytokines and chemokines related to nasal diseases were detected. Results The properties of nasal secretions collected by the cotton piece method were more uniform than the sponge method. The concentration of IL-6 in the disease group collected by the cotton piece method was significantly higher than that in the control group (P = 0.002), and the cotton piece method could distinguish the positive detection rates of IL-1β (P = 0.031) and TNF-α (P = 0.001) between the control and disease groups. The levels of inflammatory mediators in nasal secretions could preliminarily distinguish different nasal diseases. Conclusions The cotton piece method is a noninvasive and reliable method for collecting nasal secretions, which is beneficial for detecting local inflammatory and immune responses of the nasal mucosa.