Molecular profiling of behavioural development:: differential expression of mRNAs for inositol 1,4,5-trisphosphate 3-kinase isoforms in naive and experienced honeybees (Apis mellifera)
Molecular profiling of behavioural development:: differential expression of mRNAs for inositol 1,4,5-trisphosphate 3-kinase isoforms in naive and experienced honeybees (Apis mellifera)
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DOI：
10.1016/s0169-328x(01)00325-4
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发表时间：
2002-03-28
期刊：
影响因子：
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通讯作者：
Maleszka, R
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文献类型：
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作者：
Kucharski, R;Maleszka, R
在寻找可能控制成年蜜蜂延长行为成熟的遗传因素时，我们发现肌醇1.4，5-三磷酸（IP 3）3-激酶，IP 3介导的信号级联中的关键酶，在幼稚的、新出现的蜜蜂和有经验的觅食者的大脑中差异表达。DNA测序产生了一个21.5 kb的重叠群，跨越蜜蜂IP 3 K基因座和一个3'侧翼基因，类似于转录因子NFR-κ-B。IP 3 K基因座产生三种差异表达的主要转录本，它们通过选择性剪接产生，编码具有相同的高度保守的C-末端和不同的非保守的N-末端结构域的蛋白质。A型转录本在成人大脑中占主导地位，其表达水平在成人发育的前4天增加了3倍。B型信息在幼稚蜜蜂的脑中表达，但也在胸部和腹部中发现，而转录本C主要在非神经组织和触角中表达。与A型信息相反，大脑中转录本B的水平在成年后的前4天下降。我们的数据进行了评估的背景下，不成熟的和有经验的工蜂的对比行为表型。(C)2002 Elsevier Science B. V.保留所有权利。
In seeking genetic factors that may control the extended behavioural maturation of adult honeybees we found that inositol 1.4,5-trisphosphate (IP3) 3-kinase, a key enzyme in the IP3-mediated signalling cascade, is differentially expressed in brains of naive, newly emerged bees and experienced foragers. DNA sequencing yielded a contig of 21.5 kb spanning the honeybee IP3K locus and a 3' flanking gene similar to a transcription factor NFR-kappa-B. The IP3K locus gives rise to three differentially expressed major transcripts produced by alternative splicing that encode proteins with identical, highly conserved C-termini and distinct, non-conserved N-terminal domains. The type A transcript is dominant in the adult brain and its level of expression increases threefold during the first 4 days of adult development. The type B message is expressed in brains of naive bees, but is also found in the thorax and abdomen, whereas transcript C is expressed largely in non-neural tissues and in the antenna. In contrast to type A message, the brain levels of transcript B decrease during the first 4 days of adult life. Our data are evaluated in the context of the contrasting behavioural phenotypes of immature and experienced worker honeybees. (C) 2002 Elsevier Science B.V. All rights reserved.