Lycopene reduces cholesterol absorption through the downregulation of Niemann-Pick C1-like 1 in Caco-2 cells
Lycopene reduces cholesterol absorption through the downregulation of Niemann-Pick C1-like 1 in Caco-2 cells
复制标题
番茄红素通过下调 Caco-2 细胞中的 Niemann-Pick C1-like 1 来减少胆固醇吸收
DOI：
10.1002/mnfr.201500221
复制
发表时间：
2015
影响因子：
5.2
通讯作者：
Feng Dan
中科院分区：
文献类型：
--
作者：
Zou Jun;Feng Dan
血胆固醇升高是动脉粥样硬化和冠心病的重要危险因素。番茄的番茄红素被发现具有降低胆固醇的作用，并且该作用被认为与抑制胆固醇合成有关。然而，由于血浆胆固醇水平也受到肠道胆固醇吸收的影响，本研究旨在研究番茄红素是否影响肠道Caco-2细胞对胆固醇的吸收。方法和结果用不同浓度的番茄红素预处理Caco-2细胞24 h，然后与放射性胶束胆固醇孵育2 h。通过液体闪烁定量放射性胆固醇的吸收。通过Western blot和qPCR分析Niemann-Pick C1-like 1（NPC 1 L1）和肝脏X受体α（LXRα）的表达。我们发现番茄红素剂量依赖性地抑制胆固醇吸收和NPC 1 L1蛋白和NPC 1 L1 mRNA的表达。番茄红素对胆固醇吸收和NPC 1 L1表达的抑制作用可以通过阻断LXRα途径来阻止。结论这项研究首次证明番茄红素抑制肠细胞的胆固醇吸收，并且番茄红素的这种抑制作用至少部分是由LXRα-NPC 1 L1信号通路介导的。
ScopeElevated blood cholesterol is an important risk factor associated with atherosclerosis and coronary heart disease. Tomato lycopene has been found to have a hypocholesterolemic effect, and the effect was considered to be related to inhibition of cholesterol synthesis. However, since plasma cholesterol levels are also influenced by the absorption of cholesterol in the gut, the present study is to investigate whether lycopene affects cholesterol absorption in the intestinal Caco‐2 cells.Methods and resultsThe Caco‐2 cells were pretreated with lycopene at different concentrations for 24 h and then incubated with radioactive micellar cholesterol for 2 h. The absorption of radioactive cholesterol was quantified by liquid scintillation. The expression of Niemann‐Pick C1‐like 1 (NPC1L1) and liver X receptor α (LXRα) was analyzed by Western blot and qPCR. We found that lycopene dose dependently inhibited cholesterol absorption and the expression of NPC1L1 protein and NPC1L1 mRNA. The inhibitory effects of lycopene on cholesterol absorption and NPC1L1 expression could be prevented by blockade of the LXRα pathway.ConclusionThis study provides the first evidence that lycopene inhibits cholesterol absorption in the intestinal cells and this inhibitory effect of lycopene is mediated, at least in part, by LXRα‐NPC1L1 signaling pathway.