Changes of Protein Turnover in Aging Caenorhabditis elegans
Changes of Protein Turnover in Aging Caenorhabditis elegans
复制标题
DOI：
10.1074/mcp.ra117.000049
复制
发表时间：
2017-09-01
影响因子：
7
通讯作者：
Braeckman, Bart P.
中科院分区：
文献类型：
--
作者：
Dhondt, Ineke;Petyuk, Vladislav A.;Braeckman, Bart P.
蛋白质周转率在衰老的生物体中严重下降，包括C。优雅的然而，有限的信息是在个人的蛋白质水平老化过程中的营业额动态。我们使用多脉冲N-15标记和精确的质谱方法，以一天的分辨率跟踪蛋白质周转率的变化。40%的蛋白质组显示随着年龄的增长，营业额逐渐放缓，而只有少数蛋白质显示营业额增加。蛋白质周转率的下降是一致的，只有少数功能相关的蛋白质子集，包括微管蛋白和卵黄蛋白原，而随机发散的营业额模式随年龄的正常。我们的数据表明，增加异质性的翻译机制的蛋白质周转，而蛋白质周转的泛素-蛋白酶体和抗氧化剂系统是随着时间的推移保存完好。因此，我们假设，质量控制机制的维护是一种保护策略，在老化的蠕虫，虽然最终的蛋白质组崩溃是不可避免的。
Protein turnover rates severely decline in aging organisms, including C. elegans. However, limited information is available on turnover dynamics at the individual protein level during aging. We followed changes in protein turnover at one-day resolution using a multiple-pulse N-15-labeling and accurate mass spectrometry approach. Forty percent of the proteome shows gradual slowdown in turnover with age, whereas only few proteins show increased turnover. Decrease in protein turnover was consistent for only a minority of functionally related protein subsets, including tubulins and vitellogenins, whereas randomly diverging turnover patterns with age were the norm. Our data suggests increased heterogeneity of protein turnover of the translation machinery, whereas protein turnover of ubiquitin-proteasome and antioxidant systems are well-preserved over time. Hence, we presume that maintenance of quality control mechanisms is a protective strategy in aging worms, although the ultimate proteome collapse is inescapable.