Antibacterial and Larvicidal Activities of Sri Lankan Endemic Plant, Vateria copallifera
Antibacterial and Larvicidal Activities of Sri Lankan Endemic Plant, Vateria copallifera
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斯里兰卡特有植物 Vateria copallifera 的抗菌和杀幼虫活性
DOI：
10.5530/pj.2011.19.14
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发表时间：
2011
影响因子：
--
通讯作者：
K. Kulatunga
中科院分区：
文献类型：
--
作者：
S. Senadeera;C. Wijayarathna;I. Weerasinghe;K. Kulatunga
摘要研究了斯里兰卡特有植物紫菀（Vateria copallifera）的叶、树皮、果皮和种子的丙酮粗提物对10种临床重要病原微生物和致倦库蚊（Culex quinquefasciatus）和埃及伊蚊（Aedes aegypti）3龄幼虫的抑菌和杀幼虫活性。据报道，种子对甲氧西林耐药金黄色葡萄球菌（MRSA）具有最高的抗菌活性，抑菌圈为19.66 ± 0.65 mm，从最小抑菌浓度（MIC）> 0.01526 mg/ml和最小杀菌浓度（MBC）> 0.125 mg/ml可以看出。果皮对耐甲氧西林金黄色葡萄球菌的抗菌活性最低，MIC > 0.125mg/ml，MBC > 4 mg/ml。报道最高抗菌活性的种子提取物进行生物测定指导分级，并获得五个级分。(F1、F2、F3、F4、F5）。从种子提取物的五个级分中，F2报道了最高的抗菌活性。市售抗生素片磺胺甲恶唑甲氧苄啶（25 μg）、氨苄青霉素（10 μg）、红霉素（15 μg）用作阳性对照。无水乙醇为阴性对照，抑菌圈为0 ± 0。仅报告了种子提取物的杀幼虫活性，致倦库蚊的LC 50和LC 90值分别为465.3mg/ml（915.8mg.ml），埃及伊蚊的LC 50和LC 90值分别为661.3mg/ml和1105.9mg/ml。
Abstract Crude acetone extracts of leaf, bark, pericarp and seeds of Sri Lankan endemic plant, Vateria copallifera were evaluated for antibacterial and larvicidal activities against ten clinically important pathogenic microorganisms and 3 rd instar mosquito larvae of Culex quinquefasciatus and Aedes aegypti . The highest antibacterial activity with zone of inhibition 19.66 ± 0.65 mm was reported from the seeds against Methicilin resistant staphylococcus aureus (MRSA) as evident from minimum inhibitory concentration (MIC) > 0.01526 mg/ml and minimum bactericidal concentration (MBC) > 0.125 mg/ml. The lowest antibacterial activity was reported from the pericarp against Methicilin resistant staphylococcus aureus with MIC > 0.125 mg/ml and MBC > 4 mg/ml. Seed extract which reported the highest antibacterial activity was subjected to bioassay guided fractionation and obtained five fractions. (F1, F2, F3, F4, F5). From five fractions of seed extract, F2 reported the highest antibacterial activity. Commercially available antibiotic discs sulphamethoxazole trimethoprim (25 μg), ampicilin (10 μg), erythromycin (15 μg) were used as positive controls. Absolute ethanol was the negative control with zone of inhibition 0 ± 0. Larvicidal activity was reported only from seed extract with LC 50 and LC 90 values 465.3mg/ml, 915.8mg.ml for Culex quinquefasciatus and 661.3mg/ml and 1105.9mg/ml for Aedes aegypti respectively.