Uptake of iodinated contrast material in ischemic myocardium as an indicator of loss of cellular membrane integrity.
Uptake of iodinated contrast material in ischemic myocardium as an indicator of loss of cellular membrane integrity.
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缺血心肌中碘造影剂的摄取作为细胞膜完整性丧失的指标。
DOI：
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发表时间：
1980
影响因子：
6
通讯作者：
J. Newell
中科院分区：
文献类型：
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作者：
J. L. Abraham;C. Higgins;J. Newell
与正常心肌相比，含碘造影剂(I)在心肌梗死中的差异摄取已被计算机传输断层扫描（CTT）检测到。本研究采用扫描电镜和新鲜冷冻切片的能量色散x射线显微分析，观察了静脉注射造影剂后缺血性损伤犬心肌I的组织学和细胞分布。对碳基6 μ厚切片的单个细胞进行分析，发现在缺血损伤的心肌细胞中检测到I，而在正常心肌细胞中检测不到。钾钠比的下降证实了积累了I的缺血损伤细胞中膜完整性的丧失。这些结果表明，I进入了缺血损伤的心肌细胞，而不是正常的心肌细胞，这表明静脉注射造影剂后的CTT扫描可能能够确定缺血损伤后细胞失去膜完整性的心肌区域。
Differential uptake of iodine containing radiographic contrast medium (I) in myocardial infarcts compared with normal mycardium has been detected by computerized transmission tomography (CTT). In this study the histologic and cellular distribution of I in ischemically damaged canine myocardium after intravenous administration of contrast material was examined by the use of scanning electron microscopy and energy dispersive X-ray microanalysis of fresh frozen cryosections. Analysis of individual cells in 6-mu thick sections mounted on carbon substrates showed that I was detectable in the ischemically damaged but not the normal myocardial cells. A decline in the potassium-to-sodium ratio confirmed the loss of membrane integrity in the ischemically damaged cells that accumulated I. These results indicate that I enters ischemically damaged but not normal myocardial cells suggesting that CTT scans after intravenous administration of contrast material may be capable of defining the area of the myocardium in which cells have lost membrane integrity after an ischemic injury.