Genomic and functional techniques to mine the microbiome for novel antimicrobials and antimicrobial resistance genes.
Genomic and functional techniques to mine the microbiome for novel antimicrobials and antimicrobial resistance genes.
复制标题
用于挖掘新型抗微生物和抗菌抗性基因的微生物组的基因组和功能技术。
DOI：
10.1111/nyas.13257
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发表时间：
2017-01
影响因子：
5.2
通讯作者：
Dantas G
中科院分区：
文献类型：
--
作者：
Adu-Oppong B;Gasparrini AJ;Dantas G
微生物群落包含多种细菌，在每种环境中都发挥着重要的功能作用。在过去的几十年里，测序和计算方法的进步已经阐明了来自不同栖息地的微生物群落的系统发育和功能多样性。微生物组编码的功能包括合成和抵抗小分子的能力，从而产生抗菌活性。鉴于临床抗菌素耐药性的增加和抗菌素发现和批准渠道的减少所造成的公共卫生紧急情况，以及抗菌素生物合成与耐药性之间密切的生态和进化关系，这些功能特别值得关注。在这里，我们回顾了基因组和功能方法已经开发用于获取微生物组的抗菌生物合成和耐药能力，并重点介绍了其应用的杰出例子。
Microbial communities contain diverse bacteria that play important functional roles in every environment. Advances in sequencing and computational methodologies over the past decades have illuminated the phylogenetic and functional diversity of microbial communities from diverse habitats. Among the functions encoded in microbiomes are the abilities to synthesize and resist small molecules, yielding antimicrobial activity. These functions are of particular interest when viewed in light of the public health emergency posed by the increase in clinical antimicrobial resistance and the dwindling antimicrobial discovery and approval pipeline, and given the intimate ecological and evolutionary relationship between antimicrobial biosynthesis and resistance. Here, we review genomic and functional methods that have been developed for accessing the antimicrobial biosynthesis and resistance capacity of microbiomes and highlight outstanding examples of their applications.