A NOVEL METHOD FOR NUCLEIC-ACID SEQUENCE DETERMINATION
A NOVEL METHOD FOR NUCLEIC-ACID SEQUENCE DETERMINATION
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DOI：
10.1016/s0022-5193(88)80246-7
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发表时间：
1988-12-07
影响因子：
2
通讯作者：
SMITH, GC
中科院分区：
文献类型：
--
作者：
BAINS, W;SMITH, GC
我们描述了一种新的测序方法，应该容易和完全自动化。该方法依赖于将核苷酸或脱氧核苷酸序列片段化为短片段，随后通过与固体支持物上的寡脱氧核苷酸杂交来定量片段。原始序列可从所得表重构为片段频率。我们提出了一个具体的协议，这将允许实际执行这种方法。
We describe a novel sequencing methodology which should be readily and completedly automated. The method relies on fragmentation of a nucleotide or deoxynucleotide sequence into short fragments, and subsequent quantitation of the fragments by hybridization to oligo-deoxynucleotides on a solid support. The original sequence may be reconstructed from the resulting table to fragment frequencies. We present a specific protocol which would allow practical implementation of this approach.