LOCALIZATION OF THYROTROPIN-RELEASING-HORMONE PROHORMONE MESSENGER-RIBONUCLEIC-ACID IN RAT-BRAIN BY INSITU HYBRIDIZATION
LOCALIZATION OF THYROTROPIN-RELEASING-HORMONE PROHORMONE MESSENGER-RIBONUCLEIC-ACID IN RAT-BRAIN BY INSITU HYBRIDIZATION
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DOI：
10.1210/endo-121-1-98
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发表时间：
1987-07-01
期刊：
影响因子：
4.8
通讯作者：
LECHAN, RM
中科院分区：
文献类型：
--
作者：
SEGERSON, TP;HOEFLER, H;LECHAN, RM
我们研究了原TRH mRNA在大鼠脑中的分布，通过原位杂交组织化学，使用放射性标记的单链cRNA探针，以确认的假设，TRH前体分布超出区域，包含免疫反应TRH。以前被认为含有TRH的中枢神经系统的所有区域都显示出杂交。室旁核内侧小细胞分裂中的垂体神经元比前小细胞分裂细胞表现出更强烈的杂交，表明表达的区域差异。此外，以前没有认识到包含TRH在神经元胞体的免疫细胞化学的区域显示特异性杂交促TRH mRNA。这些细胞包括嗅球、迷走神经背侧运动核、中脑导水管周围灰质腹外侧、丘脑网状核和前连合核。在中脑导水管周围灰质和网状核的RNA提取物的北方印迹上仅观察到一条杂交带，与室旁核提取物中所见的杂交带相同。TRH前体mRNA在神经元中的出现以前没有被认为含有TRH，但含有激素原，这表明TRH前体中的非TRH肽可能优先在大脑的某些区域表达。
We studied the distribution of pro-TRH mRNA in rat brain by in situ hybridization histochemistry using radiolabeled single stranded cRNA probes to confirm the hypothesis that the TRH precursor is distributed beyond regions that contain immunoreactive TRH. All regions of the central nervous system previously recognized to contain TRH showed hybridization. Hypophysiotropic neurons in the medial parvocellular division of the paraventricular nucleus showed more intense hybridization than anterior parvocellular division cells, suggesting regional differences in expression. In addition, regions not previously recognized to contain TRH in neuronal perikarya by immunocytochemistry showed specific hybridization for pro-TRH mRNA. These include cells in the olfactory bulbs, dorsal motor nucleus of the vagus, ventrolateral periaqueductal gray, reticular nucleus of the thalamus, and anterior commissural nucleus. Only a single hybridizing band was observed on Northern blots of RNA extracts of the periaqueductal gray and reticular nucleus, identical to that seen in extracts of the paraventricular nucleus. The appearance of pro-TRH mRNA in neurons not previously recognized to contain TRH but which contain the prohormone suggests that non-TRH peptides within the TRH precursor may be preferentially expressed in certain regions of the brain.