Bacterial oxidation of ferrous iron at low temperatures
Bacterial oxidation of ferrous iron at low temperatures
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DOI：
10.1002/bit.21371
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发表时间：
2007-08-15
影响因子：
3.8
通讯作者：
Tuovinen, H.
中科院分区：
文献类型：
--
作者：
Kupka, Daniel;Rzhepishevska, Olena I.;Tuovinen, H.
这项研究包括第一次报告的亚铁氧化的psychrotolerant）嗜酸性铁氧化细菌能够生长在5摄氏度。将来自诺里尔斯克矿区（西伯利亚泰梅尔）和Kristineberg（瑞典乌弗勒特区）的受矿井排水影响的表层土壤和沉积物样品接种到酸性硫酸亚铁培养基中，并在5 ℃下培养。铁氧化之前约3个月的滞后期，在随后的文化减少。选择三种富集培养物用于进一步的工作，并且通过从诺里尔斯克富集培养物中的菌落分离来纯化一种指定为分离物SS 3的培养物，用于测定铁氧化的动力学。测定了SS 3和另外两种耐冷培养物（来自诺里尔斯克的SS 5和来自北方瑞典的SK 5）的基于16 S rRNA的遗传。扩增的16 S rRNA基因序列的比较分析表明，耐冷文化内的嗜酸氧化亚铁硫杆菌对齐。铁氧化的速率常数在0.0162-0.0104 h（-1）范围内，取决于初始pH。氧化动力学遵循指数模式，符合一级速率表达式。嗜酸性氧化亚铁硫杆菌的嗜温参考培养物的平行铁氧化是极其缓慢和线性的。从5 ℃培养物中收获的结晶体通过X射线衍射鉴定为施氏石（理想分子式Fe 8 O 8（OH）（6）SO 4）和黄钾铁矾（KFe 3（SO 4）（2）（OH）（6））的混合物。黄钾铁矾在30 ℃下产生的沉淀物中更占优势。
This study comprises the first report of ferrous iron oxidation by psychrotolerant) acidophilic iron-oxidizing bacteria capable of growing at 5 degrees C. Samples of mine drainage-impacted surface soils and sediments from the Norilsk mining region (Taimyr, Siberia) and Kristineberg (Skellefte district, Sweden) were inoculated into acidic ferrous sulfate media and incubated at 5 degrees C. Iron oxidation was preceded by an approximately 3-month lag period that was reduced in subsequent cultures. Three enrichment cultures were chosen for further work and one culture designated as isolate SS3 was purified by colony isolation from a Norilsk enrichment culture for determining the kinetics of iron oxidation. The 16S rRNA based phylogeny of SS3 and two other psychrotolerant cultures, SS5 from Norilsk and SK5 from Northern Sweden, was determined. Comparative analysis of amplified 16S rRNA gene sequences showed that the psychrotolerant cultures aligned within Acidithiobacillus ferrooxidans. The rate constant of iron oxidation by growing cultures of SS3 was in the range of 0.0162-0.0104 h(-1) depending on the initial PH. The oxidation kinetics followed an exponential pattern, consistent with a first order rate expression. Parallel iron oxidation by a mesophilic reference culture of Acidithiobacillus ferrooxidans was extremely slow and linear. Precipitates harvested from the 5 degrees C culture were identified by X-ray diffraction as mixtures of schwertmannite (ideal formula Fe8O8(OH)(6)SO4) and jarosite (KFe3(SO4)(2)(OH)(6)). Jarosite was much more dominant in precipitates produced at 30 degrees C.