Biochemical changes in the cervical tissue of rabbit induced by interleukin-8, interleukin-1beta, dehydroepiandrosterone sulphate and prostaglandin E2: a comparative study.
Biochemical changes in the cervical tissue of rabbit induced by interleukin-8, interleukin-1beta, dehydroepiandrosterone sulphate and prostaglandin E2: a comparative study.
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白细胞介素8、白细胞介素1β、硫酸脱氢表雄酮和前列腺素E2诱导家兔宫颈组织生化变化的比较研究。
DOI：
10.1093/oxfordjournals.humrep.a019304
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发表时间：
1996
影响因子：
6.1
通讯作者：
Toshihiko Terao
中科院分区：
文献类型：
--
作者：
E. Maradny;Naohiro Kanayama;Abdul Halim;K. Maehara;K. Sumimoto;Toshihiko Terao
本研究旨在研究和比较白细胞介素（IL）-8、IL-1 β脱氢表雄酮硫酸酯（DHEA-S）和前列腺素（PG）E2对宫颈的作用机制。用安慰剂或阴道栓剂形式的试验药物对5个相等组的妊娠家兔（n = 45）进行试验，每天一次，持续3天。栓剂含有人重组IL-8（100 ng）、IL-1 β（200 ng）、DHEA-S（10 mg）或PGE 2（1 mg）。所有家兔均进行一次、两次或三次给药试验。在末次给药后24 h估计稠度、扩张度和含水量。用抗兔RT 2单克隆抗体对宫颈组织切片进行染色后，研究宫颈的白细胞浸润。在用天狼星红染色后评估相对胶原蛋白浓度。胶原酶，明胶酶和弹性蛋白酶的活性测定在100毫克的匀浆子宫颈结缔组织。子宫颈含水量显著增加。IL-8和IL-1 β组在第二次给药后神经细胞数量增加（P分别< 0.0005和0.001）。在PGE 2组中，中性粒细胞在治疗第三剂量后增加，而在DHEA-S组中未观察到显著变化。IL-8、IL-1 β和PGE 2组治疗后胶原含量显著降低（P分别< 0.004、0.005和0.03）。DHEA-S组胶原含量在第3次给药后下降（P < 0.05）。IL-8、IL-1和DHEA-S组在第2次给药后胶原酶活性明显升高（P分别< 0.001、0.003和0.007）。PGE 2组胶原酶活性无明显升高。IL-8、IL-1 β、PGE 2和DHEA-S组第2次给药后明胶酶活性显著升高（P分别< 0.008、0.01、0.003和0.05）。此外，所有组中的弹性蛋白酶活性在第二剂量治疗后均增加（分别为P < 0.001、0.001、0.001和0.006）。我们的数据表明，兔子宫颈的成熟可以通过不同的机制启动。细胞因子在宫颈成熟中起着至关重要的作用，特别是IL-8和IL-1。IL-8是以类似于足月生理过程的方式使宫颈成熟的因素之一。
The aim of this research was to study and compare the mechanism of action of interleukin (IL)-8, IL-1beta dehydroepiandrosterone sulphate (DHEA-S) and prostaglandin (PG)E2 on the cervix. Five equal groups of pregnant rabbits (n = 45) were tested by either placebo or tested drugs in the form of vaginal suppositories once daily for 3 days. The suppositories contained human recombinant IL-8 (100 ng), IL-1beta (200 ng), DHEA-S (10 mg) or PGE2 (1 mg). All rabbits were tested by one dose, two doses or three doses. Consistency, dilatation and water contents were estimated 24 h after the last dose of treatment. Leukocyte infiltration of the cervices was studied after staining the cervical tissue sections with antirabbit RT2 monoclonal antibodies. Relative collagen concentration was assessed after staining with Picrosirius Red. Collagenase, gelatinase and elastase activities were measured in 100 mg of homogenized cervical connective tissue. Water contents were significantly increased in all tested cervices. Neutrophil numbers were increased in IL-8 and IL-1beta groups after the second dose of treatment (P < 0.0005 and 0.001 respectively). In the PGE2 group, neutrophils were increased after the third dose of treatment, whereas in DHEA-S group no significant changes were observed. Collagen content was significantly decreased in IL-8, IL-1beta and PGE2 groups after the first dose of treatment (P < 0.004, and 0.005 and 0.03 respectively). In the DHEA-S group, the decrease in collagen content occurred after the third dose (P < 0.05). Collagenase activity was markedly increased in IL-8, IL-1, and DHEA-S groups after the second dose of treatment (P < 0.001, 0.003 and 0.007 respectively). No significant increase in collagenase activity was found in PGE2 group. Gelatinase activity was significantly increased in IL-8, IL-1beta, PGE2 and DHEA-S groups after the second dose of treatment (P < 0.008, 0.01, 0.003 and 0.05 respectively). Also, elastase activity was increased after the second dose of treatment in all groups (P < 0.001, 0.001, 0.001 and 0.006 respectively). Our data suggest that ripening of the cervix in rabbit can be initiated by different mechanisms. Cytokines play a vital role in cervical ripening, especially IL-8 and IL-1. IL-8 is one of the factors which could ripen the cervix in a manner similar to the physiological process at term.
Interleukin-1 刺激人羊膜的前列腺素生物合成。
DOI：
10.1016/0090-6980(89)90028-2
发表时间：
1989
期刊：
Prostaglandins
影响因子：
--
作者：
Romero,R;Durum,S;Dinarello,CA;Oyarzun,E;Hobbins,JC;Mitchell,MD
通讯作者：
Mitchell,MD
人多形核白细胞弹性蛋白酶对人 III 型胶原蛋白的特异性裂解。
DOI：
--
发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Mainardi,CL;Hasty,DL;Seyer,JM;Kang,AH
通讯作者：
Kang,AH