Distinguishing between extra‐ and intracellular iron in marine phytoplankton.

Distinguishing between extra‐ and intracellular iron in marine phytoplankton.
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海洋浮游植物中细胞外铁和细胞内铁的区别。

DOI:
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发表时间:
1989
期刊:
影响因子:
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通讯作者:
F. Morel
F. Morel
中科院分区:
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文献类型:
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作者:
R. Hudson;F. Morel

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我们展示了柠檬酸盐和 EDTA 络合的无机还原剂钛 (III) 作为试剂在 pH 8 下快速溶解细胞外铁的用途,而不会导致细胞破损或毒性。即使存在大量沉淀的氢氧化铁,该方法也可以通过实验区分细胞外铁和细胞内铁
We demonstrate the utility of the inorganic reductant titanium (III) complexed by citrate and EDTA as a reagent for rapidly dissolving extracellular iron at pH 8 without causing cell breakage or toxicity. The method allows an experimental distinction to be made between extra- and intracellular iron, even in the presence of large quantities of precipitated iron hydroxides