IDENTIFICATION OF CUTC AND CUTF (NLPE) GENES INVOLVED IN COPPER TOLERANCE IN ESCHERICHIA-COLI
IDENTIFICATION OF CUTC AND CUTF (NLPE) GENES INVOLVED IN COPPER TOLERANCE IN ESCHERICHIA-COLI
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DOI：
10.1128/jb.177.15.4207-4215.1995
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发表时间：
1995-08-01
影响因子：
3.2
通讯作者：
WU, HC
中科院分区：
文献类型：
--
作者：
GUPTA, SD;LEE, BTO;WU, HC
以前已经提出，大肠杆菌中的铜转运由至少六个基因的产物介导，cutA、cutB、cutC、cutD、cutE和cutF。这些基因中的一个或多个突变会导致铜敏感性增加（D。Rouch、J. Camakaris和B. T. O. Lee，p. 469-477，in D. H. Hamer和D. R. Winge，编，金属离子稳态：分子生物学和化学，1989）。用大肠杆菌基因组文库转化铜敏感cutC和cutF突变体。大肠杆菌，并筛选出耐铜转化子。鉴定了两个不同的克隆，每个克隆都部分恢复了E的cutC和cutF突变体的铜耐性。杆菌亚克隆、物理定位和序列分析表明，cutC基因定位于E. cutF基因位于大肠杆菌基因组的4.77 min处，编码146个氨基酸的胞质蛋白。与Silhavy和同事CW独立鉴定的nlpE基因等位。B、斯奈德湖J. B. Davis，P. N.达内塞角L. Cosma和T. J. Silhavy，J. Bacteriol. 177：4216-4223，1995）。从cutF突变体中的铜敏感性突变的遗传作图和cutC和cutF（nlpE）从cutC和cutF突变体的等位基因的测序结果表明，cutC和cutF突变体实际上是在这两个基因中改变的双突变体，并且在这两个基因中的突变似乎是每个突变体中的铜敏感性表型所需的。
It has been suggested previously that copper transport in Escherichia coli is mediated by the products of at least six genes, cutA, cutB, cutC, cutD, cutE, and cutF. A mutation in one or more of these genes results in an increased copper sensitivity (D. Rouch, J. Camakaris, and B. T. O. Lee, p. 469-477, in D. H. Hamer and D. R. Winge, ed., Metal ion Homeostasis: Molecular Biology and Chemistry, 1989). Copper-sensitive cutC and cutF mutants were transformed with a genomic library of E. coli, and copper-tolerant transformants were selected. Two distinct clones were identified, each of which partially restores copper tolerance in both the cutC and cutF mutants of E. coli. Subcloning, physical mapping, and sequence analysis have revealed that the cutC gene is located at 42.15 min on the E. coli genome and encodes a cytoplasmic protein of 146 amino acids and that the cutF gene is located at 4.77 min on the E. coli genome and is allelic to the nlpE gene independently identified by Silhavy and coworkers CW. B, Snyder, L. J. B. Davis, P. N. Danese, C. L. Cosma, and T. J. Silhavy, J. Bacteriol. 177:4216-4223, 1995). Results from the genetic mapping of the copper-sensitive mutations in the cutF mutant and sequencing of the cutC and cutF (nlpE) alleles from both cutC and cutF mutants indicate that both the cutC and cutF mutants are in fact double mutants altered in these two genes, and mutations in both the genes appear to be required for the copper-sensitive phenotype in each mutant.