Novel thick filament protein of chicken pectoralis muscle: the 86 kd protein. I. Purification and characterization.
Novel thick filament protein of chicken pectoralis muscle: the 86 kd protein. I. Purification and characterization.
复制标题
鸡胸肌新型粗丝蛋白：86 kd蛋白。
DOI：
10.1016/0022-2836(85)90112-3
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发表时间：
1985
影响因子：
5.6
通讯作者：
T. Wallimann
中科院分区：
文献类型：
--
作者：
M. Bähler;H. Eppenberger;T. Wallimann
采用与兔骨骼肌H蛋白纯化方法相似的方法，从鸡胸大肌C蛋白粗提物中分离到一种新的粗丝相关蛋白，即86 kd蛋白。然而，通过凝胶电泳和凝胶渗透分别估计的表观Mr为86，000和370，000的蛋白质与C蛋白无关，并且通过其从羟基磷灰石柱的洗脱行为、其分子量、紫外光谱、氨基酸组成和定位以及其存在于肌原纤维中的量而不同于兔H蛋白。氨基酸组成揭示了高含量的脯氨酸和凝胶渗透表明分子的高度不对称或聚合物结构。在兔中产生的抗86 kd蛋白的抗体通过免疫双扩散和免疫印迹实验被证明是特异性的这种蛋白。它们与鸡胸大肌的肌原纤维蛋白如肌球蛋白、M带蛋白、肌联蛋白和C蛋白均无交叉反应，与兔H蛋白也无明显交叉反应。86 kd蛋白质也通过抗体亲和色谱法从洗涤的肌原纤维的新鲜制备的Guba-Straub提取物中纯化，是位于A带的每一半内的特异性肌原纤维组分。
A new thick-filament-associated protein, the 86 kd protein, of chicken pectoralis major muscle was isolated from a crude C-protein preparation by a method similar to that used to purify H-protein from rabbit skeletal muscle. However, the protein with an apparentMrof 86,000 and 370,000 as estimated by gel electrophoresis and gel permeation, respectively, is not related to C-protein and differs from rabbit H-protein by its elution behaviour from hydroxyapatite columns, by its molecular weight, ultraviolet light spectrum, amino acid composition and localization, and by its amount present in myofibrils. The amino acid composition reveals a high content of proline and gel permeation indicates an either highly asymmetric or polymeric structure of the molecule.Antibodies raised in rabbits against the 86 kd protein were demonstrated by double immunodiffusion and immunoblotting experiments to be specific for this protein. They show no cross-reactivity with any other myofibrillar protein of chicken pectoralis muscle, e.g. myosin, M-band proteins, titin or C-protein, nor did they exhibit a significant crossreactivity with H-protein from rabbit. The 86 kd protein, which has been purified also by antibody affinity chromatography from a freshly prepared Guba-Straub extract of washed myofibrils, is a specific myofibrillar component located within each half of the A-band.
影响因子：
2.9
作者：
Wallimann,T;Szent-Györgyi,AG
通讯作者：
Szent-Györgyi,AG