A critical role for phospholipase C in protective immunity conferred by listeriolysin O-deficient Listeria monocytogenes
A critical role for phospholipase C in protective immunity conferred by listeriolysin O-deficient Listeria monocytogenes
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DOI：
10.1016/j.micpath.2007.08.002
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发表时间：
2008-02-01
影响因子：
3.8
通讯作者：
Way, Sing Sing
中科院分区：
文献类型：
--
作者：
Orgun, Nural N.;Way, Sing Sing
减毒重组单核细胞增生李斯特氏菌 (Lm) 菌株是一类有前景的疫苗载体，可触发保护性抗原特异性 CD8 T 细胞。李斯特菌溶血素 0 (LLO) 是一种重要的 Lm 毒力决定因子，允许细菌从内吞液泡逃逸到吞噬细胞的细胞质中。然而，在非吞噬细胞中，Lm 磷脂酶 C 也可以介导细胞质进入。缺乏 LLO 的 Lm 能否提供长期感染保护的能力尚不确定。在此，我们证明 LLO 缺陷型 Lm 突变体可以引发对随后的 Lm 感染的保护性免疫，并且 Lm 磷脂酶 C 是 LLO 缺陷型 Lm 赋予的保护性免疫所必需的。 (C) 2007 Elsevier Ltd. 保留所有权利。
Attenuated recombinant Listeria monocytogenes (Lm) strains are a promising class of vaccine vectors that trigger protective antigen-specific CD8 T cells. Listeriolysin 0 (LLO) is an important Lm virulence determinant allowing the bacterium to escape from the endocytic vacuole into the cell cytoplasm in phagocytic cells. However in non-phagocytic cells, Lm phospholipase C can also mediate cytoplasmic entry. The ability of LLO-deficient Lm to confer long-term protection to infection is uncertain. Herein, we demonstrate that LLO-deficient Lm mutants can prime protective immunity to subsequent Lm infection and that Lm phospholipase C is required for protective immunity conferred by LLO-deficient Lm. (C) 2007 Elsevier Ltd. All rights reserved.