The two origins of hemocytes in Drosophila
The two origins of hemocytes in Drosophila
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DOI：
10.1242/dev.00702
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发表时间：
2003-10-01
期刊：
影响因子：
4.6
通讯作者：
Klapper, R
中科院分区：
文献类型：
--
作者：
Holz, A;Bossinger, B;Klapper, R
与许多其他生物一样，果蝇的血液由几种类型的血细胞组成，它们起源于中胚层。通过移植细胞的谱系分析，我们指定了两个单独的原基，产生不同的血细胞群体：胚胎血细胞和淋巴腺血细胞。胚胎血细胞的原基被限制在头部中胚层内的70%至80%卵长之间的区域。与所有其他中胚层细胞相反，这种原基的细胞在胚盘阶段已经被确定为血细胞。出乎意料的是，这些血细胞在幼虫后期不会退化，但有能力通过变态持续存在，并且在成虫中仍然可以检测到。第二原基，这产生额外的血细胞在变态的开始，位于胸中胚层内的50至53%的蛋长。在该区域内移植后，在幼虫淋巴腺中检测到克隆。标记的血细胞由淋巴腺释放，但不早于幼虫三龄晚期。这些淋巴腺来源的血细胞的原基在胚盘阶段不确定，如克隆与其他组织的重叠所示。我们的分析表明，蛹和成年苍蝇的血细胞由胚胎血细胞和淋巴腺来源的血细胞的混合物，起源于两个不同的原基，在不同的发展阶段确定。
As in many other organisms, the blood of Drosophila consists of several types of hemocytes, which originate from the mesoderm. By lineage analyses of transplanted cells, we specified two separate anlagen that give rise to different populations of hemocytes: embryonic hemocytes and lymph gland hemocytes. The anlage of the embryonic hemocytes is restricted to a region within the head mesoderm between 70 and 80% egg length. In contrast to all other mesodermal cells, the cells of this anlage are already determined as hemocytes at the blastoderm stage. Unexpectedly, these hemocytes do not degenerate during late larval stages, but have the capacity to persist through metamorphosis and are still detectable in the adult fly. A second anlage, which gives rise to additional hemocytes at the onset of metamorphosis, is located within the thoracic mesoderm at 50 to 53% egg length. After transplantation within this region, clones were detected in the larval lymph glands. Labeled hemocytes are released by the lymph glands not before the late third larval instar. The anlage of these lymph gland-derived hemocytes is not determined at the blastoderm stage, as indicated by the overlap of clones with other tissues. Our analyses reveal that the hemocytes of pupae and adult flies consist of a mixture of embryonic hemocytes and lymph gland-derived hemocytes, originating from two distinct anlagen that are determined at different stages of development.