Organ specificity, colonization and clearance dynamics in vivo following oral challenges with the murine pathogen Citrobacter rodentium
Organ specificity, colonization and clearance dynamics in vivo following oral challenges with the murine pathogen Citrobacter rodentium
复制标题
DOI：
10.1111/j.1462-5822.2004.00414.x
复制
发表时间：
2004-10-01
影响因子：
3.4
通讯作者：
Frankel, G
中科院分区：
文献类型：
--
作者：
Wiles, S;Clare, S;Frankel, G
啮齿类柠檬酸杆菌属于人类和动物肠道病原体家族，包括临床上显著的肠出血性大肠杆菌（EHEC）和肠致病性大肠杆菌。coli（EPEC）。这些病原体利用附着和消退（A/E）病变来定殖宿主胃肠道。在本研究中，我们使用生物发光成像（BLI）来研究小鼠的器官特异性，定殖和清除的动态C。啮齿类动物在现场和真实的时间。生物发光C.啮齿类衍生物菌株ICC 180表达来自病原性线虫共生体发光杆菌的luxCDABE操纵子，并且光水平准确地反映了体外和体内的细菌数量。我们已经证明了C.啮齿类动物在盲肠内发生，特别是在称为盲肠斑的淋巴组织的专门斑内。在盲肠C.啮齿动物造成了结肠感染清除C。rodentium ICC 180与定殖动力学平行，即盲肠首先被清除，然后是结肠。编码外膜粘附素intimin的生物发光基因C.啮齿动物突变体未能建立长期定殖，尽管在攻击后3天内可从盲肠回收低水平的细菌。
Citrobacter rodentium belongs to a family of human and animal enteric pathogens that includes the clinically significant enterohaemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC). These pathogens use attaching and effacing (A/E) lesions to colonize the host gastrointestinal tract. In this study we have used bioluminescence imaging (BLI) to investigate the organ specificity, dynamics of colonization and clearance of mice by C. rodentium in situ and in real time. The bioluminescent C. rodentium derivative, strain ICC180, expresses the luxCDABE operon from the entemopathogenic nematode symbiont Photorhabdus luminescens and light levels accurately reflect bacterial numbers both in vitro and in vivo. We have demonstrated that primary colonization of the mouse by C. rodentium takes place within the caecum, specifically within the specialized patch of lymphoid tissue known as the caecal patch. Following colonization of the caecum C. rodentium established a colonic infection. Clearance of C. rodentium ICC180 parallels the colonization dynamics, i.e. the caecum was first to be cleared followed by the colon. A bioluminescent eae (encoding the outer membrane adhesin intimin) C. rodentium mutant failed to establish long-term colonization, although low levels of bacteria could be recovered for up to 3 days post challenge from the caecum.